Biogenic substance detector and biogenic substance detection method

• US 7,883,901 B2
• Filed: 10/08/2008
• Issued: 02/08/2011
• Est. Priority Date: 10/26/2007
• Status: Active Grant

• Current Assignee: Seiko Epson Corporation (Seiko Group)
• Original Assignee: Seiko Epson Corporation (Seiko Group)
• Inventors: Takagi, Fumio, Kitazawa, Rie
• Primary Examiner(s): Soderquist; Arlen
• Application Number:
  US12/247,500
• Publication Number:
  US 20090111192A1
• Time in Patent Office: 853 Days
• Field of Search: 422 56- 58, 422/100, 422/102, 422/52, 436/172, 436/165
• US Class Current: 436/172
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/0086 Dimensions of the flow channels
• CPC Class Codes: B01J 2219/00952 Sensing operations
• CPC Class Codes: B01J 2219/0097 Optical sensors
• CPC Class Codes: B01J 2219/00972 Visible light
• CPC Class Codes: B01L 2300/047 Additional chamber, reservoir
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0663 Whole sensors
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 2300/163 Biocompatibility
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence

Abstract

A biogenic substance detector with high reaction efficiency and detection sensitivity is obtained.

The biogenic substance detector includes: a plurality of chambers 1011 for hybridizing a target and a probe; and a passage 1012 provided between the chambers 1011. The cross-sectional area of the passage 1012 taken perpendicularly to the chamber-passage alignment direction is smaller than the cross-sectional area of each chamber 1011 taken perpendicularly to the chamber-passage alignment direction, and a probe-fixing area 1013 is provided over the entire inside wall of each chamber 1011. One kind of probe is fixed in one chamber 1011. In the hybridization step, the sample solution is made to move back and forth within the chambers 1011 and the passage 1012 using a pump 102.

Claims

1. A biogenic substance detection method comprising:

a reaction step of supplying a sample solution to a plurality of chambers connected via a passage and having one of a plurality of biogenic substances contained in the sample solution react with one of a plurality of probes fixed in the chambers to detect the biogenic substance, a different probe being fixed in each of the chambers; and

a detection step of detecting the biogenic substance that has reacted with the one probe;

in the reaction step, the sample solution being made to move back and forth within the chambers and the passage along a chamber-passage alignment direction,

the detection step comprising the steps of;

moving a solution including a chemiluminescent enzyme back and forth within the chambers and the passage so that the chemiluminescent enzyme binds with the one of the biogenic substances that has reacted with the one of the probes;

filling the chambers and the passage with a substrate liquid that reacts the chemiluminescent enzyme to produce a chemiluminescent substance; and

resting the substrate liquid so as to produce the chemiluminescent substance, and

the passage having a first cross-sectional area taken perpendicularly to the chamber-passage alignment direction that is smaller than a second cross-sectional area of each chamber taken perpendicularly to the chamber-passage alignment direction.

2. The biogenic substance detection method according to claim 1, wherein each probe is fixed to an entire inside wall surface of each chamber.

3. The biogenic substance detection method according to claim 1, wherein the chambers and the passage are made in a transparent plate, and

wherein in the detection step, luminescence is measured through the chambers.