Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

  • US 7,892,746 B2
  • Filed: 03/16/2010
  • Issued: 02/22/2011
  • Est. Priority Date: 02/09/1996
  • Status: Expired due to Fees
First Claim
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1. A method comprising:

  • providing an array of a plurality of capture oligonucleotides wherein each type of capture oligonucleotide is greater than 16 nucleotides and differs in nucleotide sequence, when aligned to another type of capture oligonucleotide, by at least 25%, wherein said capture oligonucleotides are coupled to a solid support;

    providing a sample comprising a plurality of target oligonucleotides, each target oligonucleotide comprising (i) an addressable array-specific portion, (ii) a further nucleotide sequence, and (iii) a detectable reporter label;

    contacting the sample comprising the plurality of target oligonucleotides with the array of capture oligonucleotides under uniform hybridization conditions effective to hybridize the addressable array-specific portion of each target oligonucleotide to its complementary capture oligonucleotide; and

    detecting the reporter labels of one or more of the plurality of target oligonucleotides hybridized to their complementary capture oligonucleotides on the solid support.

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