Vitamin D deficiencies

  • US 7,901,944 B2
  • Filed: 03/26/2010
  • Issued: 03/08/2011
  • Est. Priority Date: 10/29/2004
  • Status: Active Grant
First Claim
Patent Images

1. A method for diagnosing a vitamin D deficiency or hypervitaminosis D in a mammalian patient, comprising:

  • a) obtaining a biological sample from the mammalian patient;

    b) simultaneously determining an amount of 25-hydroxyvitamin D2 and an amount of 25-hydroxyvitamin D3 in a single sample derived from the mammalian patient'"'"'s biological sample, wherein the determining comprises using a mass spectrometry technique to detect underivatized 25-hydroxyvitamin D2 and underivatized 25-hydroxyvitamin D3 in the single sample, wherein the mass spectrometry technique comprises a LC-MS/MS technique and wherein the LC-MS/MS technique comprises the use of a triple quadrupole instrument in Multiple Reaction Monitoring (MRM), positive-ion mode;

    c) comparing the amount of total 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in the single sample with clinical reference values indicative of vitamin D deficiency or hypervitaminosis D; and

    d) diagnosing either a vitamin D deficiency or hypervitaminosis D, or neither, in the mammalian patient based on the comparisons in step c).

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×