Device and methods for liquid crystal-based bioagent detection

• US 7,910,382 B2
• Filed: 10/31/2006
• Issued: 03/22/2011
• Est. Priority Date: 10/31/2005
• Status: Active Grant

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Park, Joon-Seo, Teren, Sarah, Johnson, Eric A., Beebe, David J., Abbott, Nicholas L.
• Primary Examiner(s): Cheu; Jacob
• Assistant Examiner(s): Do; Pensee T
• Application Number:
  US11/555,103
• Publication Number:
  US 20070231832A1
• Time in Patent Office: 1,603 Days
• Field of Search: None
• US Class Current: 436/518
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/37 involving peptidase or proteinase
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

The present invention provides liquid crystal-based devices and methods for bioagent detection. In certain aspects, the present invention is directed to devices and methods utilizing liquid crystals and membranes containing polymerized targets that can report the presence of bioagents including, but not limited to, enzymes, antibodies, and toxins.

Claims

1. A liquid crystal device for detecting the presence of a bioagent in a sample, comprising:

(a) a liquid crystal;

(b) an aqueous phase positioned such that an interface exists between the liquid crystal and said aqueous phase; and

(c) a membrane located at the interface containing a polymerized target of a bioagent, said polymerized target comprising a target of the bioagent covalently cross-linked to a polymer network, wherein interaction of said bioagent with the polymerized target causes an orientation change in said liquid crystal thereby indicating the presence of the bioagent in the sample.

2. The liquid crystal device according to claim 1 wherein said bioagent is an enzyme and the target is a substrate for the enzyme.

3. The liquid crystal device according to claim 1 wherein said bioagent is an antibody and the target is an antigen recognized by said antibody.

4. The liquid crystal device according to claim 1 wherein said bioagent is an antigen and the target is an antibody that recognizes said antigen.

5. The liquid crystal device according to claim 1 wherein the polymer network comprises a non-target molecule that is cross-linked to said target.

6. The liquid crystal device according to claim 5, wherein the non-target molecule is a polymer.

7. The liquid crystal device according to claim 1, wherein the aqueous phase comprises an enhancing agent that enhances the change in orientation of the liquid crystal upon interaction of the bioagent and target.

8. The liquid crystal device according to claim 7 wherein the enhancing agent is dilauroyl phosohatidylcholine (DLPC).

9. The liquid crystal device according to claim 1, further comprising a cross-linking agent.

10. A liquid crystal device for detecting the presence of a bioagent in a sample, comprising:

(a) a liquid crystal;

(b) an aqueous phase positioned such that an interface exists between the liquid crystal and said aqueous phase; and

(c) a polymerized membrane comprising a polymer cross-linked to a target of a bioagent, said polymerized membrane located at the interface between the liquid crystal and said aqueous phase wherein interaction of said bioagent with the target causes an orientation change in said liquid crystal thereby indicating the presence of the bioagent in the sample.