Analysis kit for living organism and chemical reaction

• US 7,985,384 B2
• Filed: 06/15/2007
• Issued: 07/26/2011
• Est. Priority Date: 06/19/2006
• Status: Expired due to Fees

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Yazawa, Yoshiaki, Uchida, Kenko, Anazawa, Takashi
• Primary Examiner(s): BROWN, MELANIE YU
• Application Number:
  US11/812,156
• Publication Number:
  US 20070292897A1
• Time in Patent Office: 1,502 Days
• Field of Search: 422/50, 422/400, 422/401, 422/402, 422/420, 436/518, 436/164, 436/169, 436/172, 435/7.1, 435/283.1, 435/287.1, 435/287.2, 435/287.7, 435/288.7
• US Class Current: 422/420
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387

Abstract

There is provided a simple, inexpensive reaction analysis kit and system capable of performing highly sensitive, quantitative measurement. A chemical luminescence reaction is employed and a sensor element is used to detect the reaction in a highly sensitive manner. That is, a reaction detection plate is used to transmit a signal detected in the sensor element via a reader coil and a reader and then analyze the reaction. The reaction detection plate has a) a membrane, b) a first antibody impregnated section that is disposed such that it faces the membrane and holds a first labeled antibody that specifically binds to a substance to be analyzed, c) a second antibody immobilized section that is provided in part of the membrane and has an immobilized second antibody, the second antibody specifically binding to the substance to be analyzed, and d) a sensor element that is disposed such that it faces the second antibody immobilized section and includes a light detector and a signal transceiver.

Claims

1. A reaction analysis system comprising:

a reaction detection plate including a) a membrane, b) a first antibody impregnated section that is disposed such that it faces the membrane and holds a first labeled antibody that specifically binds to a substance to be analyzed, c) a second antibody immobilized section that is disposed in part of the membrane and has an immobilized second antibody, the second antibody specifically binding to the substance to be analyzed, d) a sensor element that is disposed such that it faces the second antibody immobilized section and includes a light detector and a signal transceiver, the sensor element being disposed in close contact with the second antibody immobilized section, and e) an absorbing section disposed such that the absorbing section faces the membrane and absorbs the sample,

wherein the sensor element detects a chemical luminescence generated by a reaction between a labeling substance of the first labeled antibody and a reagent that reacts with the labeling substance and emits light;

a shielding container that houses the reaction detection plate, the shielding container having a sample introduction section for introducing a sample into the reaction detection plate,

wherein the second antibody immobilized section is disposed between the sample introduction section and the absorbing section;

a reader coil that is disposed outside the shielding container, the reader coil transmitting and receiving a signal to and from the sensor element and supplying power to the sensor element;

a reader that performs demodulation/modulation, decoding/coding and amplification of the signal; and

a processor for executing an application program that controls the sensor element.

2. The reaction analysis system according to claim 1 further comprising a sensor element-side coil in the shielding container.

3. The reaction analysis system according to claim 2 further comprising a relay coil that inductively couples the sensor element-side coil to the reader coil.

4. The reaction analysis system according to claim 1, wherein the number of the sensor element is at least two.