Sample chamber

• US 8,001,857 B2
• Filed: 06/20/2008
• Issued: 08/23/2011
• Est. Priority Date: 06/25/2007
• Status: Active Grant

• Current Assignee: ibidi GmbH
• Original Assignee: ibidi GmbH
• Inventors: Kahl, Valentin
• Primary Examiner(s): NOLAND, THOMAS
• Application Number:
  US12/143,678
• Publication Number:
  US 20080314167A1
• Time in Patent Office: 1,159 Days
• Field of Search: 738/645.1, 738/649.1, 250/576, 356/246, 422/102, 422939-940, 422942-944, 422/547, 422/549, 422/550, 422/552, 422/559, 422/FOR.110, 4353051-3054
• US Class Current: 73/864.91
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/161 Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/15 Preventing contamination of the components of the optical system or obstruction of the light path
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides

Abstract

A sample chamber of plastics has at least one receiving area for receiving a liquid, and includes an oleophobic layer in a predetermined surface area. The sample chamber includes a base plate and a cover plate firmly connected thereto. The oleophobic layer is disposed on a side of the base plate and the cover plate facing away from the receiving areas, and/or the sample chamber consists of a plastic body and the oleophobic layer is disposed on a side of the plastic body facing away from the receiving areas.

Claims

1. Sample chamber of plastics with at least two receiving areas for receiving a liquid, wherein the sample chamber comprises an oleophobic layer in a predetermined surface area between the at least two receiving areas,

wherein the sample chamber comprises a base plate and a cover plate firmly connected thereto and wherein the oleophobic layer is disposed on at least one of a side of the base plate facing away from the receiving areas, and a side of the cover plate facing away from the receiving areas.

2. Sample chamber according to claim 1, wherein the oleophobic layer has a surface tension of less than 25 mN/m.

3. Sample chamber according to claim 1, wherein the oleophobic layer has a layer thickness of 1 nm to 50 μ

m.

4. Sample chamber according to claim 1, wherein the oleophobic layer has an area of 1 μ

m² to 10 cm².

5. Sample chamber according to claim 1, wherein the base plate comprises at least one of

at least one depression, and

at least one elevation,

by which a receiving area is formed.

6. Sample chamber according to claim 1, wherein a depression is provided in the base plate, so that a receiving area is formed by the base plate and the cover plate.

7. Sample chamber according to claim 6, wherein the depression in the base plate comprises a base, so that a hollow space is formed by the cover plate.

8. Sample chamber according to claim 1, wherein the cover plate has a thickness of 50-250 μ

m.

9. Sample chamber according to claim 1, further comprising an additional predetermined surface area, including at least one of a hydrophilic layer and a second oleophobic layer.

10. Sample chamber of plastics with at least one receiving area for receiving a liquid, wherein the sample chamber comprises an oleophobic layer in a predetermined surface area,

wherein the sample chamber comprises a base plate and a cover plate firmly connected thereto and wherein the oleophobic layer is disposed all-over at least one of a side of the base plate facing away from the receiving area, and a side of the cover plate facing away from the receiving area.

11. Sample chamber according to claim 10, wherein the oleophobic layer has a surface tension of less than 25 mN/m.

12. Sample chamber according to claim 10, wherein the oleophobic layer has a layer thickness of 1 nm to 50 μ

m.

13. Sample chamber according to claim 10, wherein the base plate comprises

at least one of

at least one depression, and

at least one elevation,

by which a receiving area is formed.

14. Sample chamber according to claim 10, wherein a depression is provided in the base plate, so that a receiving area is formed by the base plate and the cover plate.

15. Sample chamber according to claim 14, wherein the depression in the base plate comprises a base, so that a hollow space is formed by the cover plate.

16. Sample chamber according to claim 10, wherein at least one of the base plate and the cover plate comprises a channel coming from outside and ending in the receiving area.

17. Sample chamber according to claim 10, wherein the cover plate has a thickness of 50-250 μ

m.

18. Sample chamber according to claim 10, wherein a double layer of a hydrophilic layer and an oleophobic layer is provided in the predetermined surface area.

19. Sample chamber according to claim 10, wherein a plurality of oleophobic layers which are spatially separated is provided.

20. Sample chamber according to claim 10, further comprising a lattice.

21. Sample chamber according to claim 20, wherein the lattice is arranged in the area of a receiving means.

22. Sample chamber according to claim 20, wherein the lattice is an embossed lattice.

23. Sample chamber according to claim 22, wherein the sample chamber comprises at least one of a base plate and a cover plate, and the lattice is embossed into the at least one of the base plate and the cover plate.

24. Sample chamber according to claim 20, wherein the lattice is applied by embossing according to a process selected from the group consisting of hot embossing, lasing, and imprinting.

25. Sample chamber according to claim 10, further comprising an additional predetermined surface area, including at least one of a hydrophilic layer and a second oleophobic layer.

26. Sample chamber according to claim 25, wherein the additional predetermined surface area is arranged in the at least one receiving area.

27. Sample chamber according to claim 25, wherein the at least one of a hydrophilic layer and a second oleophobic layer is disposed on at least one of the side of the base plate facing the at least one receiving area and the side of the cover plate facing the at least one receiving area, and is disposed across the entire surface of the at least one of the side of the base plate facing the at least one receiving area and the side of the cover plate facing the at least one receiving area, except for the receiving area.