Device and method for multiple analyte detection
First Claim
1. A device comprising:
- a substrate;
a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the two or more detection chambers and the inlet; and
non-immobilized detection reagents pre-loaded in each of the two or more detection chambers, the detection reagents comprising a first oligonucleotide, a second oligonucleotide, and a detection reagent capable of producing an optical signal;
wherein the fluid communication comprises a channel and respective fluid connections connecting the channel to the respective detection chambers of the two or more detection chambers, and the fluid connections branch off from opposite sides of the channel.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
112 Citations
45 Claims
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1. A device comprising:
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a substrate; a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the two or more detection chambers and the inlet; and non-immobilized detection reagents pre-loaded in each of the two or more detection chambers, the detection reagents comprising a first oligonucleotide, a second oligonucleotide, and a detection reagent capable of producing an optical signal; wherein the fluid communication comprises a channel and respective fluid connections connecting the channel to the respective detection chambers of the two or more detection chambers, and the fluid connections branch off from opposite sides of the channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A device comprising:
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a substrate; a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the two or more detection chambers and the inlet; and detection reagents pre-loaded in each of the two or more detection chambers; wherein the fluid communication comprises a channel and respective fluid connections connecting the channel to the respective detection chambers of the two or more detection chambers, the fluid connections branch off from opposite sides of the channel, the fluid communication comprises a sample delivery channel, and the device further comprises a plurality of dead-end fluid connections, wherein each of the dead-end fluid connections provides a dead-end communication between a respective one of the two or more detection chambers and the sample delivery channel. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
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Specification