Fourier scattering methods for encoding microbeads and methods and apparatus for reading the same
First Claim
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1. A method for reading microbeads having different respective codes, comprising:
- providing a plurality of microbeads in a random manner onto a support substrate, the microbeads being oriented in a predetermined manner on the support substrate, wherein each of the microbeads has an individually identifiable code that can be projected on and read from a Fourier plane;
illuminating the microbeads with an incident light, the codes providing corresponding output light signals indicative of the respective codes, wherein the corresponding output light signal from each of the microbeads is projected individually on the Fourier plane, wherein only one microbead is illuminated at a time by the incident light to read the respective code; and
reading the respective codes from the Fourier plane.
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Abstract
A method, optical arrangement, and apparatus for reading a microbead having a code. The method includes reflecting an incident light from the microbead to provide an output light. The output light is projected on a Fourier plane. The method also includes reading the code from the Fourier plane.
453 Citations
40 Claims
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1. A method for reading microbeads having different respective codes, comprising:
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providing a plurality of microbeads in a random manner onto a support substrate, the microbeads being oriented in a predetermined manner on the support substrate, wherein each of the microbeads has an individually identifiable code that can be projected on and read from a Fourier plane; illuminating the microbeads with an incident light, the codes providing corresponding output light signals indicative of the respective codes, wherein the corresponding output light signal from each of the microbeads is projected individually on the Fourier plane, wherein only one microbead is illuminated at a time by the incident light to read the respective code; and reading the respective codes from the Fourier plane. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. An optical system for reading microbeads having different respective codes, the optical system comprising:
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a support substrate configured to receive a plurality of microbeads in a random manner, the microbeads being oriented in a predetermined manner on the support substrate, wherein each of the microbeads has an individually identifiable code that can be projected on and read from a Fourier plane; an input light source configured to direct an incident light onto the codes of the microbeads to provide an output light signal from each of the microbeads that is projected individually on the Fourier plane, the output light signals being indicative of the respective codes, wherein only one microbead is illuminated at a time by the incident light to read the respective code; and a detector that is configured to detect the corresponding output light signal from each of the microbeads from the Fourier plane. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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36. A method for reading a code from an encoded microbead, the method comprising:
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providing an encoded microbead, the microbead having a chemical probe immobilized thereto, wherein the microbead has an individually identifiable code that is correlated to the chemical probe immobilized thereto, the code configured to be projected on and read from the Fourier plane; illuminating the microbead with an incident light thereby providing an output light signal, the output light signal being projected onto a Fourier plane; performing Fourier plane analysis of the output light signal projected onto the Fourier plane to determine a code therefrom; identifying the chemical probe using the code; and determining whether the chemical probe has selectively bound to a target analyte after the microbead is exposed to the target analyte. - View Dependent Claims (37, 38, 39)
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40. An apparatus for reading encoded microbeads, each microbead providing output light signals projected individually onto a Fourier plane when illuminated by an incident light, the apparatus comprising:
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a support substrate configured to receive a plurality of microbeads in a random manner, the microbeads being oriented in a predetermined manner on the support substrate, wherein each of the microbeads has an individually identifiable code that can be projected on and read from the Fourier plane; a reading device positioned to detect the corresponding output light signal of each microbead from the Fourier plane, wherein the reading device is configured to read the corresponding output light signal of each microbead one at a time; and a processor configured to perform Fourier plane analysis of the output light signals projected onto the Fourier plane to determine different respective codes of the encoded microbeads.
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Specification