Methods and compositions for activating regenerative stem cells from bone marrow

• US 8,093,048 B2
• Filed: 12/07/2007
• Issued: 01/10/2012
• Est. Priority Date: 06/28/2001
• Status: Active Grant

• Current Assignee: Rongxiang Xu
• Original Assignee: Rongxiang Xu
• Inventors: Xu, Rongxiang
• Primary Examiner(s): Weber, Jon P
• Assistant Examiner(s): UNDERDAHL, THANE E
• Application Number:
  US11/952,904
• Publication Number:
  US 20080085322A1
• Time in Patent Office: 1,495 Days
• Field of Search: 435/377, 512/25
• US Class Current: 435/377
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/20 having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
• CPC Class Codes: A61K 31/201 having one or two double bonds, e.g. oleic, linoleic acids
• CPC Class Codes: A61K 31/56 Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
• CPC Class Codes: A61K 31/575 substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
• CPC Class Codes: A61K 35/644 Beeswax; Propolis; Royal jelly; Honey
• CPC Class Codes: A61K 36/15 Pinaceae (Pine family), e.g. pine or cedar
• CPC Class Codes: A61K 36/185 Magnoliopsida (dicotyledons)
• CPC Class Codes: A61K 36/286 Carthamus (distaff thistle)
• CPC Class Codes: A61K 36/48 Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
• CPC Class Codes: A61K 36/539 Scutellaria (skullcap)
• CPC Class Codes: A61K 36/61 Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
• CPC Class Codes: A61K 36/63 Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
• CPC Class Codes: A61K 36/738 Rosa (rose)
• CPC Class Codes: A61K 36/756 Phellodendron, e.g. corktree
• CPC Class Codes: A61K 36/82 Theaceae (Tea family), e.g. camellia
• CPC Class Codes: A61K 36/899 Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 17/14 : for baldness or alopecia
• CPC Class Codes: A61P 17/16 : Emollients or protectives, e.g. against radiation
• CPC Class Codes: A61P 19/00 : Drugs for skeletal disorders
• CPC Class Codes: A61P 25/28 : for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61P 31/04 : Antibacterial agents
• CPC Class Codes: A61P 31/12 : Antivirals
• CPC Class Codes: A61P 35/00 : Antineoplastic agents
• CPC Class Codes: A61P 7/02 : Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors

Abstract

The present invention provides novel compositions and methods for pharmaceutical or nutraceutical use in an animal, preferably in a human. In one aspect, compositions and methods are provided for activating regenerative stem cells from bone marrow, which can lead to enhanced proliferation of hematopoietic cells and immune functions of the body.

Claims

1. A method for activating the bone marrow of a subject for wound repair comprising:

releasing bone marrow from the bone adjacent to the site of a wound in the subject; and

topically administering to the site of the wound with released bone marrow a composition comprising beeswax at a concentration of 1%-20% by weight and a fatty acid-containing oil at a concentration of at least 10% by weight based on the total weight of the composition; and

a sterol compound at a concentration of at least 1% by weight based on the total weight of the composition.

2. The method of claim 1, wherein the fatty acid-containing oil is at a concentration at least 50% by weight based on the total weight of the composition.

3. The method according to claim 1, wherein the fatty acid-containing oil is at a concentration at least 80% by weight based on the total weight of the composition.

4. The method according to claim 1, wherein the concentration of the sterol compound is about 1.2-40% by weight.

5. The method according to claim 1, wherein the concentration of the sterol compound is about 1.2-20% by weight.

6. The method according to claim 1, wherein the concentration of the sterol compound is about 1.5-15% by weight.

7. The method according to claim 1, wherein the concentration of the sterol compound is about 2-6% by weight.

8. The method according to claim 1, wherein the composition further comprises baicalin.

9. The method according to claim 1, wherein the composition further comprises an extract of huangqin in an amount of 10-90% by weight based on the total weight of the composition, wherein the amount of huangqin is 2-60% by weight based on the total weight of the oil.

10. The method according to claim 1, wherein the oil is animal or vegetable oil.

11. The method according to claim 1, wherein the oil is vegetable oil selected from the group consisting of corn oil, peanut oil, cottonseed oil, rice bran oil, safflower oil, tea tree oil, pine nut oil, macadamia nut oil, camellia seed oil, rose hip oil, sesame oil, olive oil, soybean oil and combinations thereof.

12. The method according to claim 1, wherein the oil is a pharmaceutically acceptable oil.

13. The method according to claim 1, wherein the oil is an injectable oil.

14. The method according to claim 1, wherein the fatty acid is selected from the group consisting of palmitic acid, linoleic acid, oleic acid, trans-oleic acid, stearic acid, arachidic acid, and tetracosanoic acid.

15. The method according to claim 1, wherein the sterol compound is animal sterol or phytosterol.

16. The method according to claim 1, wherein the sterol compound is a phytosterol selected from the group consisting of stigmasterol, campesterol, β

-sitosterol, chalinosterol, clionasterol, brassicasterol, α

-spinasterol, daucosterol, desmosterol, avenasterol, cycloartenol, poriferasterol, and natural or synthesized, isomeric forms and derivatives thereof.

17. The method according to claim 1, wherein the sterol compound is a combination of stigmasterol and β

-sitosterol.

18. The method according to claim 1, wherein the sterol compound is a combination of brassicasterol and β

-sitosterol.

19. The method according to claim 1, wherein the sterol compound is a combination of brassicasterol, stigmasterol and β

-sitosterol.

20. The method according to claim 1, wherein the sterol compound is a combination of campesterol, stigmasterol and β

-sitosterol.