Analysis instrument

• US 8,101,137 B2
• Filed: 02/08/2006
• Issued: 01/24/2012
• Est. Priority Date: 02/08/2005
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Lab901 Limited (Agilent Technologies, Inc.)
• Inventors: Polwart, Stuart, Macnamara, Kenneth G., Lamb, Urs, Fearnley, Joel
• Primary Examiner(s): Barton, Jeffrey T
• Assistant Examiner(s): DIETERLE, JENNIFER M
• Application Number:
  US11/815,852
• Publication Number:
  US 20100126857A1
• Time in Patent Office: 2,176 Days
• Field of Search: 204600-650, 204/450, 204/453, 422/100, 422/99, 422400-430, 422500-570, 422/82, 738/649.1
• US Class Current: 422/504
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 2035/1034 Transferring microquantities of liquid
• CPC Class Codes: G01N 35/00009 provided with a sample supporting tape, e.g. with absorbent zones

Abstract

An analysis instrument for processing a microfluidic device, having sample storage means, a microfluidic device holder, sample loading means for loading sample into a microfluidic device disposed in the holder, processing means for enabling a reaction in a microfluidic device, and detection means for detecting and/or measuring the reaction is disclosed. The microfluidic device holder is adapted to hold the microfluidic device including a tape in position for processing and/or detection. A microfluidic processing device is also disclosed which includes a reaction chamber, and a sample loading chamber into which a sample is injectable. The reaction chamber is operatively connected to the sample loading chamber. A cover extends across at least part of the sample loading chamber. The cover and the reaction chamber include pierceable material and are separated by an overspill cavity configured to accept any overspill of an injected sample. A kit is also disclosed which has the analysis instrument and the microfluidic processing device as described above.

Claims

1. A microfluidic processing device comprising:

a reaction chamber;

a carrier layer comprising piercable material;

a sample loading chamber into which a sample is injectable;

a piercable process layer; and

an overspill cavity;

wherein the sample loading chamber is formed between the reaction chamber, which is operatively connected to the sample loading chamber, and the piercable process layer; and

wherein the overspill cavity is formed from a space between the piercable process layer and the carrier layer and configured to accept any overspill.

2. A microfluidic processing device according to claim 1, wherein the carrier layer and the piercable process layer are components of a laminated structure.

3. A microfluidic processing device according to claim 1, wherein the reaction chamber comprises a molecular separation medium.

4. A microfluidic processing device according to claim 3, wherein the carrier layer and/or the piercable process layer are manufactured from polymer film.

5. A microfluidic processing device according to claim 3, including optical fiducial marks having known positions relative to the reaction chamber and which can be acquired by a detection means of an analysis instrument to accurately identify a position of a reaction process.

6. A microfluidic processing device according to claim 3, wherein the reaction chamber is a channel.

7. A microfluidic processing device according to claim 6, wherein the microfluidic processing device further includes a receiving chamber at an end of the reaction channel remote from the sample loading chamber.