Fluorescent nanoscopy method

  • US 8,110,405 B2
  • Filed: 09/27/2010
  • Issued: 02/07/2012
  • Est. Priority Date: 05/18/2005
  • Status: Expired due to Fees
First Claim
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1. A method for nanoscopy of objects, comprising:

  • exciting a fluorescent agent introduced within an object;

    registering the excited fluorescent agent as a plurality of separate spots with a detection device;

    losing the color of the fluorescent agents;

    repeating a cycle of the exciting and registering steps a plurality of times;

    using the spots for calculation coordinates of the fluorescent agents; and

    creating an image with a data processor from the coordinates of the fluorescent agents registered as spots in the repeated cycles.

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