Microwave-assisted extraction of polysaccharide from Artemisia sphaerocephala

  • US 8,110,677 B2
  • Filed: 02/24/2009
  • Issued: 02/07/2012
  • Est. Priority Date: 02/26/2008
  • Status: Expired due to Fees
First Claim
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1. A process for obtaining purified Artemisia sphaerocephala polysaccharide with microwave-assistance, comprising:

  • a) contacting Artemisia sphaerocephala seed powder with supercritical CO2 to obtain an oil-extracted powder;

    contacting the oil-extracted powder with distilled water at a ratio of 1;

    30 to 1;

    50 (w/w) to obtain a suspension;

    exposing the suspension to microwaves of a frequency of 915 MHz and overall power of 600 W to 800 W in a microwave extraction apparatus comprising a water condenser, thereby obtaining an extractant;

    filtering the extractant at a pressure of 0.07 to 0.09 MPa to obtain a filtrate;

    subjecting the filtrate to a pressure of 0.06 to 0.08 MPa to reduce the total volume of the filtrate by ¾

    to ⅞

    , thereby obtaining a concentrate;

    contacting the concentrate with ethanol to obtain a concentrated extract which comprises about 70 to 80% ethanol (v/v);

    centrifuging the concentrated extract at 3500 to 4000 rpm to obtain a precipitate; and

    subjecting the precipitate to a pressure of 1 Pa and a temperature of −

    60 to −

    50°

    C. for 24 to 36 hours to obtain material A;

    b) i) contacting material A with distilled water at a ratio of 1;

    150 to 1;

    200 (w/v) to obtain an aqueous solution;

    ii) heating the aqueous solution at a temperature of 60 to 70°

    C. and then incubating with 6μ

    -8μ

    /100ml papain for 10 to 12 hours;

    iii) contacting the aqueous solution with a preparation of butanol and chloroform wherein the butanol;

    chloroform ratio is 1;

    2 to 1;

    4 (v/v);

    iv) recovering the aqueous phase;

    v) repeating steps iii) and iv) a further 3 to 6 times to prepare a final aqueous phase;

    vi) subjecting the final aqueous phase to a pressure of 0.06 to 0.08 MPa and a temperature of 40 to 50°

    C. to collect and concentrate the aqueous phase to ⅛

    to ¼

    of original volume, thereby obtaining material B; and

    c) contacting material B with ammonia to obtain a solution of pH 9;

    contacting the solution of pH 9 with a preparation of 20 to 30% H2O2 to obtain a colorless solution;

    subjecting the colorless solution to a pressure of 0.06 to 0.08 Mpa, thereby obtaining a concentrated solution;

    contacting one side of a semi-permeable membrane with the concentrated solution and another side of the semi-permeable membrane with distilled water, wherein the semi-permeable membrane has an average molecular weight cut-off of 8000 to 14000 Da, for 36 to 48 hours;

    adding ethanol to the dialyzed preparation to obtain an extract solution which comprises about 70 to 80% ethanol (v/v);

    centrifuging the extract solution at 3500 to 4000 rpm to collect a precipitate; and

    subjecting the collected precipitate to a pressure of 1 Pa and a temperature of −

    60 to −

    50°

    C. for 24 to 36 hours, thereby obtaining purified Artemisia sphaerocephala polysaccharide.

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