Human TNF receptor

  • US 8,163,522 B1
  • Filed: 05/19/1995
  • Issued: 04/24/2012
  • Est. Priority Date: 09/12/1989
  • Status: Active Grant
First Claim
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1. A method comprising the steps of:

  • (a) culturing a host cell comprising a polynucleotide, wherein the polynucleotide encodes a protein consisting of;

    (i) the extracellular region of an insoluble human TNF receptor, wherein the insoluble human TNF receptor has an apparent molecular weight of about 75 kilodaltons as determined on a non-reducing SDS-polyacrylamide gel and comprises the amino acid sequence LPAQVAFXPYAPEPGSTC (SEQ ID NO;

         10), and(ii) all of the domains of the constant region of a human IgG immunoglobulin heavy chain other than the first domain of said constant region, and(b) purifying an expression product of the polynucleotide from the cell mass or the culture medium.

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