Method of manufacturing a disposable electrochemical sensor strip

• US 8,187,446 B2
• Filed: 04/19/2005
• Issued: 05/29/2012
• Est. Priority Date: 06/17/2003
• Status: Active Grant

• Current Assignee: Chun-Mu Huang
• Original Assignee: Chun-Mu Huang
• Inventors: Huang, Chun-Mu
• Primary Examiner(s): Olsen, Kaj K
• Application Number:
  US11/109,169
• Publication Number:
  US 20050187097A1
• Time in Patent Office: 2,597 Days
• Field of Search: 205/159, 205/264, 205/777.5, 204/403.01, 204/403.02
• US Class Current: 205/159
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A disposable electrochemical sensor strip is provided. The sensor strip includes an isolating sheet having at least a through hole, at least a conductive raw material mounted in the through hole, a metal film covered on the conductive raw material to form an electrode which comprises an electrode working surface for processing an electrode action, and an electrode connecting surface, at least a printed conductive film mounted on the isolating sheet and having a connecting terminal for being electrically connected to the electrode connecting surface, and a signal output terminal for outputting a measured signal produced by the electrode action.

Claims

1. A method for manufacturing a disposable electrochemical sensor strip, comprising steps of:

providing an isolating piece having a length from a first end to a second end, a first side, a second side, a recess and at least one through hole, wherein the at least one through hole is on the first end of the isolating piece and has a first opening having a size on the first side and a second opening on the second side, and the recess extends from the second opening along the length on the second side to the second end;

preparing a conductive assembly comprising a conductive raw material;

coating a metal film on said conductive raw material for forming a modified electrode having an electrode working surface for processing an electrode action, an output terminal and a modified electrode body between the working surface and the output terminal; and

mounting the working surface of said modified electrode in said through hole to place the working surface in the first opening such that an electrode working area of the working surface is equal to the size of the first opening, the output terminal toward the second end on the second side, and the body in the recess,

wherein a current measured by the disposable electrochemical sensor strip is proportional to said electrode working area.

2. The method according to claim 1 further comprising a step of electroplating said metal film on said conductive raw material for modifying said first conductive raw material to obtain said modified electrode.

3. The method according to claim 1 further comprising a step of immobilizing an enzyme on said electrode for modifying said electrode to obtain an enzyme electrode.

4. The method according to claim 1, wherein said working surface is revealed on said isolating piece by tightly engaging said modified electrode within said through hole and contacts with a fluid sample.

5. The method according to claim 4 further comprising a step of forming a flowing channel on said isolating piece, wherein said flowing channel, is configured to allow the fluid sample to flow therein and directly contact said working surface such that an analyte in the fluid sample may react with a reagent disposed on said working surface.

6. A method for manufacturing a disposable electrochemical sensor strip, comprising steps of:

providing an isolating piece and a conductive raw material;

defining on the isolating piece at least a through hole, a first side, a second side, a length from a first end to a second end and a recess, the through hole having a first opening with a geometric shape on the first side and a second opening on the second side, the recess extending from the second opening to the second end;

coating a metal film on the conductive raw material for forming a modified electrode;

mounting the modified electrode in the through hole to form an electrode working surface by the geometric shape;

printing a conductive material at the second end on the second side to form an output terminal; and

connecting the modified electrode through the recess to the output terminal,

wherein a current measured by the disposable electrochemical sensor strip is proportional to the electrode working surface.