Methods and compositions for the detection of biological molecules using a two particle complex

CAFC
  • US 8,188,243 B2
  • Filed: 06/23/2005
  • Issued: 05/29/2012
  • Est. Priority Date: 06/23/2004
  • Status: Active Grant
First Claim
Patent Images

1. A composition for detecting an analyte of interest in a sample comprising:

  • (A)k, (B)u, (C), (D)xwherein A is an ECL moiety which can be induced to repeatedly emit electromagnetic radiation by direct exposure to an electrochemical energy source;

    B is at least one first carrier containing A, wherein A can be released from B and B is either linked to(1) an analog of the analyte of interest;

    or(2) to a first specific binding partner of the analyte of interest;

    C is the sample which may contain the analyte of interest; and

    D is at least one second carrier and is either linked to(3) the analog of the analyte of interest;

    or(4) a second specific binding partner of the analyte of interest;

    wherein k is an integer equal to or greater than 2; and

    u and x are each an integer equal to or greater than 1,provided that B and D are not both linked to the analog of the analyte of interest, and provided that if B is linked to the analog of the analyte of interest, then D is linked to the second specific binding partner and if D is linked to the analog of the analyte of interest, then B is linked to the first specific binding partner,wherein B comprises an interior and includes a plurality of the ECL moieties A within the interior of B; and

    the ECL moiety A is soluble in organic solvent and insoluble in aqueous solvent.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×