Methods and compositions for the detection of biological molecules using a two particle complex
DC CAFCFirst Claim
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1. A composition for detecting an analyte of interest in a sample comprising:
- (A)k, (B)u, (C), (D)xwherein A is an ECL moiety which can be induced to repeatedly emit electromagnetic radiation by direct exposure to an electrochemical energy source;
B is at least one first carrier containing A, wherein A can be released from B and B is either linked to(1) an analog of the analyte of interest;
or(2) to a first specific binding partner of the analyte of interest;
C is the sample which may contain the analyte of interest; and
D is at least one second carrier and is either linked to(3) the analog of the analyte of interest;
or(4) a second specific binding partner of the analyte of interest;
wherein k is an integer equal to or greater than 2; and
u and x are each an integer equal to or greater than 1,provided that B and D are not both linked to the analog of the analyte of interest, and provided that if B is linked to the analog of the analyte of interest, then D is linked to the second specific binding partner and if D is linked to the analog of the analyte of interest, then B is linked to the first specific binding partner,wherein B comprises an interior and includes a plurality of the ECL moieties A within the interior of B; and
the ECL moiety A is soluble in organic solvent and insoluble in aqueous solvent.
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Abstract
The invention provides methods of detecting analytes of interest in a sample using electrogenerated chemiluminescence. The invention also provides compositions comprising at least one solid support that entraps or contains an electrogenerated chemiluminescent moiety.
33 Citations
50 Claims
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1. A composition for detecting an analyte of interest in a sample comprising:
- (A)k, (B)u, (C), (D)x
wherein A is an ECL moiety which can be induced to repeatedly emit electromagnetic radiation by direct exposure to an electrochemical energy source; B is at least one first carrier containing A, wherein A can be released from B and B is either linked to (1) an analog of the analyte of interest;
or(2) to a first specific binding partner of the analyte of interest; C is the sample which may contain the analyte of interest; and D is at least one second carrier and is either linked to (3) the analog of the analyte of interest;
or(4) a second specific binding partner of the analyte of interest; wherein k is an integer equal to or greater than 2; and
u and x are each an integer equal to or greater than 1,provided that B and D are not both linked to the analog of the analyte of interest, and provided that if B is linked to the analog of the analyte of interest, then D is linked to the second specific binding partner and if D is linked to the analog of the analyte of interest, then B is linked to the first specific binding partner, wherein B comprises an interior and includes a plurality of the ECL moieties A within the interior of B; and
the ECL moiety A is soluble in organic solvent and insoluble in aqueous solvent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 29, 31, 32, 33, 34, 35, 38, 39, 46, 47, 48, 49, 50)
- (A)k, (B)u, (C), (D)x
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21. A kit for detecting an analyte of interest in a sample, comprising an ECL moiety which can be induced to repeatedly emit electromagnetic radiation by direct exposure to an electrochemical energy source;
- at least one first carrier containing the ECL moiety, wherein the ECL moiety can be released from the first carrier and the first carrier is either linked to (1) an analog of the analyte of interest;
or (2) a first specific binding partner of the analyte of interest;
at least one second carrier which is either linked to (3) the analog of the analyte of interest;
or (4) a second specific binding partner of the analyte of interest;
provided that the first carrier and the second carrier are not both linked to the analog of the analyte of interest, and provided that if the first carrier is linked to the analog of the analyte of interest, then the second carrier is linked to the second specific binding partner and if the second carrier is linked to the analog of the analyte of interest, then the first carrier is linked to the first specific binding partner,wherein the first carrier comprises an interior and a plurality of the ECL moieties are contained within the interior of the first carrier; and
the ECL moieties are soluble in organic solvent and insoluble in aqueous solvent. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 30, 36, 37, 40, 41, 42, 43, 44, 45)
- at least one first carrier containing the ECL moiety, wherein the ECL moiety can be released from the first carrier and the first carrier is either linked to (1) an analog of the analyte of interest;
Specification