Substrate for biochip, biochip, method for manufacturing substrate for biochip, and method for manufacturing biochip
First Claim
Patent Images
1. A method for manufacturing a biochip including:
- preparing a base plate;
forming a metallic membrane on the base plate;
forming a crosslinkable polymer membrane on the metallic membrane;
selectively removing portions of the polymer membrane;
delineating a plurality of wells reaching the base plate in the metallic membrane by using the polymer membrane as an etching mask;
introducing a plurality of hydroxyl groups on a surface of the base plate exposed from the plurality of wells;
bonding a plurality of probe biomolecules including protected amino groups to the plurality of hydroxyl groups; and
soaking the base plate, the metallic membrane and the polymer membrane in an alkaline solution to deprotect the amino groups included in the plurality of probe biomolecules and peel off the polymer membrane from the metallic membrane after the probe biomolecules are introduced on the base plate,wherein the crosslinkable polymer membrane protects the metallic membrane while the probe biomolecules are introduced on the plate exposed from the plurality of wells.
1 Assignment
0 Petitions
Accused Products
Abstract
A base plate having a surface on which a plurality of hydroxyl groups can be introduced, a metallic membrane disposed on the base plate and having a plurality of wells reaching the base plate, and a crosslinkable polymer membrane disposed on the metallic membrane are included.
65 Citations
14 Claims
-
1. A method for manufacturing a biochip including:
-
preparing a base plate; forming a metallic membrane on the base plate; forming a crosslinkable polymer membrane on the metallic membrane; selectively removing portions of the polymer membrane; delineating a plurality of wells reaching the base plate in the metallic membrane by using the polymer membrane as an etching mask; introducing a plurality of hydroxyl groups on a surface of the base plate exposed from the plurality of wells; bonding a plurality of probe biomolecules including protected amino groups to the plurality of hydroxyl groups; and soaking the base plate, the metallic membrane and the polymer membrane in an alkaline solution to deprotect the amino groups included in the plurality of probe biomolecules and peel off the polymer membrane from the metallic membrane after the probe biomolecules are introduced on the base plate, wherein the crosslinkable polymer membrane protects the metallic membrane while the probe biomolecules are introduced on the plate exposed from the plurality of wells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
-
-
9. A method for manufacturing a biochip including:
-
preparing a substrate for the biochip comprising a base plate, a metallic membrane disposed on the base plate and having a plurality of wells reaching the base plate, a crosslinkable polymer membrane disposed on the metallic membrane, and a plurality of hydroxyl groups introduced on a surface of the base plate exposed from the plurality of wells; bonding a plurality of probe biomolecules including protected amino groups to the plurality of hydroxyl groups; and soaking the base plate, the metallic membrane and the polymer membrane in an alkaline solution to deprotect amino groups included in the plurality of probe biomolecules and peel off the polymer membrane from the metallic membrane after the probe biomolecules are introduced on the base plate, wherein the crosslinkable polymer membrane protects the metallic membrane while the probe biomolecules are introduced on the plate exposed from the plurality of wells. - View Dependent Claims (10, 11, 12, 13, 14)
-
Specification