Ruggedized apparatus for analysis of nucleic acid and proteins

• US 8,206,974 B2
• Filed: 05/19/2005
• Issued: 06/26/2012
• Est. Priority Date: 05/19/2005
• Status: Active Grant

• Current Assignee: ANDE Corp.
• Original Assignee: NetBio, Inc.
• Inventors: Tan, Eugene, Lam, Heung Chuan, Kellogg, Greg
• Primary Examiner(s): CROW, ROBERT THOMAS
• Application Number:
  US11/132,712
• Publication Number:
  US 20060260941A1
• Time in Patent Office: 2,595 Days
• Field of Search: 435/6, 435/6.1, 435/283.1, 435/287.2, 204/450, 204/452
• US Class Current: 435/287.2
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.

Claims

1. An apparatus for analyzing a sample of biomolecular analyte using electrophoresis, the apparatus comprising:

a test module comprising at least one microfluidic channel disposed within a member formed of a transparent material, wherein the at least one microfluidic channel is configured for electrical communication with an anode and a cathode, wherein the at least one microfluidic channel comprises a separation portion, a waste arm in fluid communication with a waste arm opening, and a sample arm in fluid communication with a sample arm opening, wherein the waste arm opening is in fluid communication with a single separation portion;

a holder configured to support the test module;

an electrophoresis device connected to the holder, comprising an anode and a cathode, which, when in use, applies one or more biasing configurations to the test module;

a light source which, when in use, emits a light beam that is transmitted to the test module and excites fluorescence in the sample of biomolecular analyte;

a light detector configured to detect the fluorescence;

a plurality of optical devices configured to transmit the light beam emitted by the light source to the test module and the fluorescence from the test module to the light detector; and

a base plate onto which the light source, the light detector, and the plurality of optical devices are secured, wherein the baseplate is supported by a frame including a dampening device adapted to reduce transmission of vibrations generated below the frame to the base plate.

2. The apparatus of claim 1, wherein the test module comprises more than one microfluidic channel.

3. The apparatus of claim 1, further comprising a heater configured to provide thermal energy to the test module.

4. apparatus of claim 1, wherein the light source draws less than 10 amps at 240 volts.

5. The apparatus of claim 1, further comprising a portable energy source to provide energy to the apparatus.

6. The apparatus of claim 5, wherein the portable energy source has a maximum power consumption of about 1.5 kVA or less than 1.5 kVA.

9. The apparatus of claim 1, wherein the base plate includes a plurality of securing elements for limiting rotational movement of the light source, the light detector, and the plurality of optical devices on the base plate.

10. The apparatus of claim 1, wherein the electrophoresis device, when in use, applies at least two biasing configurations to the test module.

13. The apparatus of claim 1, wherein the base plate includes a plurality of securing elements for limiting the rotation movement of the plurality of optical devices on the base plate.

7. An apparatus for analyzing a sample of biomolecular analyte, the apparatus comprising:

a test module comprising at least one microfluidic channel disposed within a member formed of a transparent material, wherein the at least one microfluidic channel is configured for electrical communication with an anode and a cathode, wherein the at least one microfluidic channel comprises a separation portion, a waste arm in fluid communication with a waste arm opening, and a sample arm in fluid communication with a sample arm opening, wherein the waste arm opening is in fluid communication with a single separation portion;

a holder configured to support the transparent test module;

an electrophoresis device connected to the holder, comprising an anode and a cathode, which, when in use, applies one or more biasing configurations to the test module;

a light source which, when in use, emits a light beam that is transmitted to the transparent test module and excites fluorescence in the sample of biomolecular analyte;

a light detector that detects the fluorescence;

an optical train comprising a plurality of optical devices configured to transmit the light beam emitted by the light source to the test module and the fluorescence from the test module to the light detector;

a base plate onto which the light source, the light detector, and the plurality of optical devices are secured, wherein the baseplate is supported by a frame including a dampening device adapted to reduce transmission of vibrations generated below the frame to the base plate; and

a lanefinder component for automatically determining a location of each channel.

8. The apparatus of claim 7, wherein the lanefinder component automatically locates the middle of each channel.

11. The apparatus of claim 7, wherein the base plate includes a plurality of securing elements for limiting rotational movement of the light source, the light detector, and the plurality of optical devices on the base plate.

12. The apparatus of claim 7, wherein the electrophoresis device, when in use, applies at least two biasing configurations to the test module.

14. The apparatus of claim 7, wherein the base plate includes a plurality of securing elements for limiting the rotation movement of the plurality of optical devices on the base plate.

15. A method of determining center channel locations in a transparent test module using the apparatus of claim 7, the method comprising:

providing a transparent test module including the plurality of separate fluid channels, each of the separate fluid channels having a front end position, a center channel position, and a known channel width;

scanning over a portion of the transparent test module including each of the plurality of separate fluid channels with a light beam to generate a reflected light beam;

collecting the reflected light beam with a light detector to generate a waveform of intensity through the portion of the transparent test module including each of the plurality of separate fluid channels;

eliminating peaks determined not to be associated with the plurality of separate fluid channels;

identifying a location within the waveform of the front end position for each of the plurality of separate fluid channels;

identifying all remaining peaks along the waveform within a first distance extending from each of the identified front end positions; and

determining the center channel positions of each of the plurality of separate fluid channels from an average of locations of all of the remaining peaks within the first distance from each of the identified front end positions.