Photo-damage method for sorting particles

  • US 8,206,987 B2
  • Filed: 05/12/2011
  • Issued: 06/26/2012
  • Est. Priority Date: 03/28/2003
  • Status: Active Grant
First Claim
Patent Images

1. A method of sorting a mixture of stained sperm cells having either characteristic A or characteristic B into at least one population, the method comprising the steps of:

  • a. flowing a fluid stream containing stained sperm cells through a flow path at a fluid delivery rate;

    b. exciting fluorescence emissions from the stained sperm cells having characteristic A and the stained sperm cells having characteristic B flowing in the flow path;

    c. detecting the fluorescence emissions from the excited sperm cells;

    d. classifying the stained sperm cells as either having characteristic A or having characteristic B based upon the fluorescence emissions;

    e. selecting stained sperm cells in the flow path based on their classification; and

    f. photo-damaging the selected sperm cells to produce an enriched population of sperm with respect to either characteristic A or characteristic B.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×