Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof

  • US 8,241,878 B2
  • Filed: 11/15/2011
  • Issued: 08/14/2012
  • Est. Priority Date: 09/29/2008
  • Status: Active Grant
First Claim
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1. A method of converting 2,3-dihydroxyisovalerate to α

  • -ketoisovalerate, comprising providing a recombinant yeast host cell expressing a heterologous dihydroxy-acid dehydratase (DHAD) protein that comprises(i) an amino acid sequence having at least 95% identity to SEQ ID NO;

    179 or 187; and

    (ii) three conserved cysteine residues that correspond to positions 56, 129, and 201 of SEQ ID NO;

    179;

    wherein the substrate 2,3-dihydroxyisovalerate is present in the yeast host cell and wherein the expressed heterologous DHAD protein catalyzes the conversion of 2,3-dihydroxyisovalerate to α

    -ketoisovalerate.

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