OCT4 and SOX2 with SV40 T antigen produce pluripotent stem cells from primate somatic cells

  • US 8,268,620 B2
  • Filed: 10/23/2009
  • Issued: 09/18/2012
  • Est. Priority Date: 10/24/2008
  • Status: Active Grant
First Claim
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1. A method of reprogramming primate somatic cells, the method comprising the steps of:

  • introducing into a primate somatic cell at least one non-viral episomal vector that encodes at least one potency-determining factor under conditions sufficient to reprogram the cells, wherein the at least one vector encodes at least potency-determining factors OCT4 and SOX2, wherein the cells express the introduced OCT4 and SOX2 and further comprise SV40 T Antigen; and

    culturing the cells obtained from the introducing step in a medium that supports pluripotent cell growth to obtain pluripotent reprogrammed cells substantially free of any vector component associated with introducing the potency-determining factors to the somatic cells.

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