Patterned spot microarray using photocatalyst and method of manufacturing the same

• US 8,273,533 B2
• Filed: 02/01/2007
• Issued: 09/25/2012
• Est. Priority Date: 07/25/2006
• Status: Expired due to Fees

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Chung, Jong-suk, Hwang, Kyu-youn, Shim, Jeo-young
• Primary Examiner(s): MUMMERT, STEPHANIE KANE
• Application Number:
  US11/670,207
• Publication Number:
  US 20080124719A1
• Time in Patent Office: 2,063 Days
• Field of Search: None
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/0043 for direct application of reagents, e.g. through openings in a shutter
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00599 Solution-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B81B 2201/06 Bio-MEMS
• CPC Class Codes: B81C 1/00206 Processes for functionalising a surface, e.g. provide the surface with specific mechanical, chemical or biological properties
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 50/18 using a particular method of attachment to the solid support

Abstract

Provided is a method of preparing a patterned spot microarray using a photocatalyst. The method comprises coating the photocatalyst on a substrate to form a photocatalyst layer, coating a composition comprising a functional group to be connected to a biomolecule on the photocatalyst layer to form an organic layer, spotting the biomolecule on the organic layer, positioning a photomask above a spot of the biomolecule; and irradiating the spot through the photomask to pattern the spot.

Claims

1. A method of preparing a patterned spot microarray comprising:

coating a photocatalyst on a substrate to form a photocatalyst layer;

coating a composition on the photocatalyst layer to form an organic thin film layer coated on the photocatalyst layer, wherein the organic thin film layer comprises a functional group to be connected to a nucleic acid;

spotting the nucleic acid on the organic thin film layer and immobilizing the nucleic acid;

positioning a photomask above a spot of the immobilized nucleic acid; and

irradiating the spot of the immobilized nucleic acid with ultraviolet light through the photomask to form a pattern on the spot of the immobilized nucleic acid,

wherein nucleic acid irradiated with light through the photomask is oxidized to reduce the size of the nucleic acid spot, and

wherein target nucleic acid present in a sample does not hybridize to the patterned spot microarray in an area where the nucleic acid has been irradiated with light through the photomask.

2. The method of claim 1, wherein the photocatalyst comprises TiO₂, ZnO, SnO₂, SrTiO₃, WO₃, B₂O₃, or Fe₂O₃.

3. The method of claim 1, wherein the photocatalyst layer is formed using chemical vapor deposition (CVD), physical vapor deposition (PVD), sputtering or a sol-gel method.

4. The method of claim 1, wherein the functional group to be connected to the nucleic acid comprises an amino group, a carboxyl group, an epoxy group, or a sulfur group.

5. The method of claim 1, wherein the composition comprises -aminopropyltriethoxy silane (GAPS).

6. The method of claim 1, wherein the photocatalyst layer has a thickness such that constructive interference occurs between first reflected light of irradiated excitation light reflected from the substrate and second reflected light of irradiated excitation light reflected from the photocatalyst layer.

7. The method of claim 6, wherein the photocatalyst layer has a thickness in the range of about 350 to about 1100 Å

.

8. The method of claim 1, wherein a diameter of the patterned spot of the immobilized nucleic acid is about 100 μ

m or less.

9. The method of claim 1, wherein a shape of a pattern of the patterned spot of the immobilized nucleic acid is a circle, a lozenge, a regular tetragon, a rectangle or a star.

10. The method of claim 1, wherein the substrate is glass, a silicon wafer, fused silica, gold, silver, copper, platinum, polystyrene, poly(methacrylate), or polycarbonate.

11. The method of claim 1, wherein the nucleic acid is selected from DNA, RNA or peptide nucleic acid.

12. The method of claim 1, wherein target nucleic acid hybridizes to the patterned spot microarray in an area where the nucleic acid has not been irradiated with light through the photomask.