Fiber optic based detection of autofluorescent bacterial pathogens

• US 8,280,471 B2
• Filed: 12/12/2007
• Issued: 10/02/2012
• Est. Priority Date: 12/12/2007
• Status: Active Grant

• Current Assignee: Avent Incorporated (Avanos Medical, Inc.)
• Original Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Inventors: Rainone, Mike, Phillips, Erica M., Hantke, Richard, Feaster, Shawn R., Baird, Daniel, Plowman, Thomas Edward, Presley, Talbot
• Primary Examiner(s): Winakur, Eric
• Assistant Examiner(s): Liu, Chu Chuan (JJ)
• Application Number:
  US11/954,867
• Publication Number:
  US 20090156943A1
• Time in Patent Office: 1,756 Days
• Field of Search: 600/310, 600/314, 600316-317, 600/322, 600/326, 600341-342, 600/344, 600476-478, 436 63- 64
• US Class Current: 600/310
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/445 Evaluating skin irritation or skin trauma, e.g. rash, eczema, wound, bed sore
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 2201/0221 Portable; cableless; compact; hand-held
• CPC Class Codes: G01N 2201/129 Using chemometrical methods

Abstract

Disclosed are methods and devices for continuous in vivo monitoring of a potential bacterial infection site. Disclosed devices may be utilized to alert patients and/or health care providers to the presence of pathogenic bacteria at an early stage of a hospital acquired infection, thereby providing for earlier intervention. Disclosed methods utilize optical fibers to deliver an excitation signal to an area in which pathogenic bacteria may exist. In the presence of the excitation signal, bacterial pathogens may autofluoresce with a unique spectral signature. Upon generation of a fluorescent emission, an optically detectable emission signal may be transmitted to a detection/analysis device. Analysis of the characteristics of the emission signal produced in response to the excitation signal may be used to determine the presence or concentration of pathogens at the site of inquiry, following which real time information may be transmitted to medical personnel via a wireless transmission system.

Claims

1. A method for detecting the presence or amount of a pathogenic bacterium that is a source of a hospital acquired infection comprising:

locating the end of an optical fiber in a wound or surgical site;

maintaining the end of the optical fiber in the wound or surgical site throughout a healing process of the wound or surgical site;

transmitting an excitation signal through a fiber optic cable to the wound or surgical site;

contacting any bacterial pathogen present with the excitation signal;

transmitting an autofluorescent emission signal from the bacterial pathogen to a portable detector; and

determining the presence or amount of the pathogenic bacterium that is a source of a hospital acquired infection in the wound or surgical site based on the autofluorescent emission signal.

2. The method according to claim 1, further comprising transmitting information regarding the presence or amount of the pathogenic bacterium in the wound or surgical site to a receiver.

3. The method according to claim 2, wherein the information is transmitted to the receiver by use of a wireless transmission system.

4. The method according to claim 1, further comprising locating the fiber optic cable in an in vivo environment in conjunction with a medical device.

5. The method according to claim 1, further comprising converting the autofluorescent emission signal to at least one of a visual and an audible signal.

6. The method according to claim 1, wherein the fiber optic cable contains a first optical fiber for transmitting the excitation signal and a second optical fiber for transmitting the autofluorescent emission signal.

7. The method according to claim 1, further comprising determining the presence or concentration of a second bacterium in the environment.

8. The method according to claim 1, wherein a signaling device emits a signal upon determination that the amount of the pathogenic bacterium has exceeded a concentration of about 10⁵ colony forming units per millimeter.

9. A portable device for detecting the amount of a pathogenic bacterium that is a source of a hospital acquired infection comprising:

a portable enclosure containing a power source, an excitation energy source, an optical detector, a signal processor, and a signaling device for emitting a signal upon detection of the pathogenic bacterium in a wound or surgical site, wherein the signaling device emits a signal upon detection of a bacterial concentration of the pathogenic bacterium greater than about 10⁵ colony forming units per millimeter;

a connecting device for attaching the enclosure to the clothing or body of a wearer; and

a fiber optic cable for inserting into the wound or surgical site, the fiber optic cable being in optical communication with the excitation energy source, the fiber optic cable extending for a length exterior to the enclosure, the fiber optic cable including an end configured to be maintained within the wound or surgical site throughout a healing process.

10. The device of claim 9, the enclosure further including a transmitter for transmitting a signal containing information regarding the amount of the bacteria in the wound or surgical site to a receiver.

11. The device of claim 10, wherein the transmitter is a wireless transmitter.

12. The device of claim 9, wherein the fiber optic cable contains only a single optical fiber.

13. The device of claim 9, wherein the fiber optic cable contains a plurality of optical fibers.

14. The device of claim 13, the fiber optic cable comprising a first optical fiber in optical communication with the excitation energy source, and a second optical fiber in optical communication with the optical detector.

15. The device of claim 9, the device further comprising a second fiber optic cable in optical communication with the optical detector.

16. The device of claim 9, wherein the excitation source delivers a single excitation wavelength to the fiber optic cable.

17. The device of claim 9, wherein the excitation source delivers multiple excitation wavelengths to the fiber optic cable.

18. The device of claim 9, wherein the signaling device emits a plurality of signals upon detection of the pathogenic bacterium in an environment.

19. The device of claim 9, wherein the connecting device is for connecting the enclosure to a piece of clothing.

20. The device of claim 9, wherein the connecting device comprises an adhesive.

21. The device of claim 20, wherein the connecting device is for connecting the enclosure to a wearer'"'"'s skin.

22. The device of claim 13, the device further comprising a plurality of notch filters.