Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

CAFC
  • US 8,288,521 B2
  • Filed: 03/25/2011
  • Issued: 10/16/2012
  • Est. Priority Date: 02/09/1996
  • Status: Expired due to Fees
First Claim
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1. A kit for identifying one or more of a plurality of sequences differing by single-base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences comprising:

  • a ligase;

    a collection of oligonucleotide probe sets, each set characterized by (a) a first oligonucleotide probe comprising a target-specific portion and (b) a second oligonucleotide probe comprising a target-specific portion and a further portion, wherein the further portion comprises a nucleic acid sequence of greater than sixteen nucleotides that differs for each different target-specific portion, and wherein the nucleic acid sequence of a complement to one further portion differs from the nucleic acid sequence of a complement to another further portion in the collection by at least 25%, when aligned to each other.

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