Biological specimen collection and transport system and methods of use

  • US 8,293,467 B2
  • Filed: 12/20/2011
  • Issued: 10/23/2012
  • Est. Priority Date: 10/01/2007
  • Status: Active Grant
First Claim
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1. A method for denaturing proteins, inactivating nucleases and killing pathogens in one step without degrading nucleic acid of a biological sample containing proteins, nucleases, and nucleic acid, and suspected to contain pathogens, comprising:

  • providing a mixture containing one or more chaotropes, one or more detergents, one or more reducing agents, one or more chelators, and one or more buffers, together present in an amount sufficient to denature proteins, inactivate nucleases, kill pathogens, and not degrade nucleic acid;

    contacting the biological sample with the mixture which denatures proteins, inactivate nucleases, kill pathogens, and does not degrade nucleic acid of the biological sample; and

    detecting the presence and identity of or absence of the pathogens in the biological sample.

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