Chip assay having improved efficiency

• US 8,329,010 B2
• Filed: 05/28/2008
• Issued: 12/11/2012
• Est. Priority Date: 05/03/2000
• Status: Active Grant

• Current Assignee: GeneFluidics, Inc.
• Original Assignee: Kotura, Inc. (NVIDIA Corporation)
• Inventors: Gau, Jen-Jr
• Primary Examiner(s): Barton, Jeffrey T
• Assistant Examiner(s): ROSENWALD, STEVEN ERIC
• Application Number:
  US12/154,971
• Publication Number:
  US 20090011952A1
• Time in Patent Office: 1,658 Days
• Field of Search: 204/409, 204/450, 204/453, 204/454, 204/600, 204/604, 20440301-40315, 205/777.5, 205/778, 205/792
• US Class Current: 204/403.13
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The chip includes electrodes on a substrate. The electrodes include a working electrode, a reference electrode, and a counter electrode. The reference electrode is constructed so as to not have an intrinsic potential. A self-assembly monolayer is positioned on the reference electrode. The self-assembly monolayer includes spacers and active probes. The active probes are configured to have a higher affinity for a capture probe than the spacers have for the capture probe.

Claims

1. An electrochemical sensor device, comprising:

electrodes on a substrate, the electrodes including a working electrode, a reference electrode, and a counter electrode; and

a self-assembly monolayer on the reference electrode, the self-assembly monolayer comprising 12-mercapto(8-biotinamide-3,6-dioxaoctyl)dodecanamide and 11-mercapto-1-undecanol.

2. The device of claim 1, wherein the 12-mercapto(8-biotinamide-3,6-dioxaoctyl)dodecanamide is bonded to streptavidin.

3. The device of claim 1, wherein the monolayer includes sulfur of the 12-mercapto(8-biotinamide-3,6-dioxaoctyl)dodecanamide linked directly to the reference electrode.

4. The device of claim 1, wherein the reference electrode consists of a single layer of metal selected from the group consisting of gold, silver, copper, platinum, chromium, aluminum, titanium, and nickel.

5. The device of claim 1, wherein the reference electrode consists of a single layer of gold.

6. The device of claim 1, wherein the reference electrode, the working electrode, and the counter electrode each consists of a single layer of metal selected from the group consisting of gold, silver, copper, platinum, chromium, aluminum, titanium, and nickel.

7. The device of claim 1, wherein the reference electrode, the working electrode, and the counter electrode each consists of a single layer of gold.

8. The device of claim 1, wherein the reference electrode and the counter electrode each have a shape that is different from a shape of the working electrode.

9. The device of claim 1, wherein the sensor occupies an area of 160 μ

m² to 25 mm².

10. The device of claim 1, wherein the reference electrode, the working electrode and the counter electrode each consist of a single layer of an electrically conducting material.

11. The device of claim 1, wherein the self-assembly monolayer is on the working electrode.

12. The device of claim 1, wherein the reference electrode is arranged about the perimeter of the working electrode such that a portion of the working electrode is positioned between different regions of the reference electrode.

13. The device of claim 1, wherein the counter electrode is arranged about the perimeter of the working electrode such that a portion of the working electrode is positioned between different regions of the counter electrode.