Device and methods for liquid crystal-based bioagent detection

• US 8,329,423 B2
• Filed: 01/21/2011
• Issued: 12/11/2012
• Est. Priority Date: 10/31/2005
• Status: Active Grant

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Abbott, Nicholas L., Park, Joon-Seo, Teren, Sarah, Beebe, David J., Johnson, Eric A.
• Primary Examiner(s): Shibuya, Mark
• Assistant Examiner(s): DO, PENSEE T
• Application Number:
  US13/011,514
• Publication Number:
  US 20110183357A1
• Time in Patent Office: 690 Days
• Field of Search: 435/7.9
• US Class Current: 435/7.9
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/37 involving peptidase or proteinase
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

The present invention provides liquid crystal-based devices and methods for bioagent detection. In certain aspects, the present invention is directed to devices and methods utilizing liquid crystals and membranes containing polymerized targets that can report the presence of bioagents including, but not limited to, enzymes, antibodies, and toxins.

Claims

1. A method for detecting the presence of a bioagent in a sample, comprising:

(a) forming a membrane containing a polymerized target for the bioagent at an interface between a liquid crystal and an aqueous phase, said polymerized target comprising the target for the bioagent covalently cross-linked to a polymer network;

(b) introducing the sample into the aqueous phase; and

(c) observing the orientational order of the liquid crystal, thereby detecting the presence of the bioagent in the sample, wherein interaction of the bioagent and said polymerized target leads to a change in the orientational order of the liquid crystal, said orientational change indicating the presence of the bioagent.

2. The method according to claim 1 wherein said bioagent is an enzyme and the target is a substrate for the enzyme.

3. The method according to claim 1 wherein said bioagent is an antibody and the target is an antigen recognized by said antibody.

4. The method according to claim 1 wherein said bioagent is an antigen and the target is an antibody that recognizes said antigen.

5. The method according to claim 1 wherein the polymer network comprises a non-target molecule that is cross-linked to said target.

6. The method according to claim 1 wherein said membrane is further comprised by an adsorbed or deposited layer of non-target molecules.

7. The method according to claim 1 wherein said aqueous phase includes an enhancing agent that enhances the change in orientation of the liquid crystal upon interaction of the bioagent and target.

8. The method according to claim 7 wherein the enhancing agent is L-a-dilauroyl phosphatidylcholine (L-DLPC).