Rationally-designed meganuclease variants of lig-34 and I-crei for maize genome engineering
First Claim
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1. A rationally-designed meganuclease having the amino acid sequence of SEQ ID NO:
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Abstract
The invention relates to the field of molecular biology and recombinant nucleic acid technology. In particular, the invention relates to a rationally-designed, non-naturally-occurring meganuclease with altered DNA recognition sequence specificity which recognizes and cleaves a unique DNA site in the maize genome. Disclosed herein are meganucleases which are variants of the I-CreI and LIG-34meganucleases. The invention also relates to methods of producing engineered maize plants using such meganucleases.
83 Citations
3 Claims
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1. A rationally-designed meganuclease having the amino acid sequence of SEQ ID NO:
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2. A rationally-designed meganuclease having the amino acid sequence of SEQ ID NO:
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3. A rationally-designed meganuclease having the amino acid sequence of SEQ ID NO:
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Specification
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Current AssigneePrecision BioSciences Incorporated
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Original AssigneePrecision BioSciences Incorporated
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InventorsJantz, Derek, Smith, James Jefferson
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Primary Examiner(s)Worley, Cathy Kingdon
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Application NumberUS12/879,428Publication NumberTime in Patent Office837 DaysField of Search435/6, 435/69.1, 435/199, 435/320.1, 435/325, 435/23.2, 800/278US Class Current435/199CPC Class CodesC12N 9/22 Ribonucleases RNAses, DNAse...
