Flow cytometric apparatus and method
First Claim
1. A flow cytometric apparatus for testing a sample, comprising:
- a sample storage that is to store the sample for testing;
a flow chamber that is to test the sample that is operatively connected to the sample storage;
a single fluid driving device operatively connected to the sample storage and a sheath fluid storage means to drive both the sample and a sheath fluid for testing the sample, whereinthe single fluid driving device is characterized by performing both an injection action and a pipetting action, andthe single fluid driving device switches between a sample flow path and a sheath flow path for charging or discharging the sample or the sheath fluid; and
a control means that is to control the injection action and the pipetting action.
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Accused Products
Abstract
Various embodiments disclose a flow cytometric apparatus comprising a flow chamber to be passed through by a sample in a row in sequence, a sample storage means for metering and storing the sample, a sample syringe for driving the sample into the flow chamber, a syringe-type injection-suction device comprising a sheath liquid syringe and a liquid-path switching means connected with it, and a control unit for controlling the operations of the sample syringe and the syringe-type the injection-suction means. In a sample charging stage, the injection-suction means feeds the sample into the sample storage means so that the sample is temporarily stored in it in a ready-to-use state, and in a sheath-liquid injection stage, the injection-suction means feeds the sheath liquid into the flow chamber to form a sheath flow. A flow cytometric method performed by the flow cytometric apparatus is also disclosed.
6 Citations
20 Claims
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1. A flow cytometric apparatus for testing a sample, comprising:
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a sample storage that is to store the sample for testing; a flow chamber that is to test the sample that is operatively connected to the sample storage; a single fluid driving device operatively connected to the sample storage and a sheath fluid storage means to drive both the sample and a sheath fluid for testing the sample, wherein the single fluid driving device is characterized by performing both an injection action and a pipetting action, and the single fluid driving device switches between a sample flow path and a sheath flow path for charging or discharging the sample or the sheath fluid; and a control means that is to control the injection action and the pipetting action. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method for testing a sample, comprising:
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preparing the sample by causing a single fluid driving device for performing an injection action or a pipetting action on the sample to deliver the sample from a sample storage means into a sample preparation means, wherein the injection action creates a positive pressure, and the pipetting action creates a negative pressure; injecting a sheath fluid by causing the single fluid driving device to inject or to pipet the sheath fluid into a flow chamber where the sample is to be tested; injecting the sample from the sample preparation means into the flow chamber; and testing the sample in the flow chamber. - View Dependent Claims (13, 14, 16, 17, 18, 19, 20)
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15. The method of 13, further comprising:
using the over-pressure protection means to locate a location of a blockage, wherein the blockage causes the one or more fluid paths to be over-pressurized.
Specification