Mass spectroscopy techniques for detecting vitamin D compounds in a sample

  • US 8,349,613 B2
  • Filed: 02/07/2011
  • Issued: 01/08/2013
  • Est. Priority Date: 10/29/2004
  • Status: Active Grant
First Claim
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1. A mass spectroscopy method for monitoring a vitamin D replacement therapy in a mammalian patient comprising:

  • a) obtaining a biological sample from the mammalian patient, wherein the mammalian patient has received at least one dose of a vitamin D replacement therapy;

    b) determining an amount of 25-hydroxyvitamin D2 in a sample derived from the mammalian patient'"'"'s biological sample, wherein the determining comprises using a mass spectrometry technique to detect underivatized 25-hydroxyvitamin D2, wherein the mass spectrometry technique comprises a LC-MS/MS technique and wherein the LC-MS/MS technique comprises the use of a triple quadrupole instrument in Multiple Reaction Monitoring (MRM), positive ion mode; and

    c) comparing the amount of 25-hydroxyvitamin D2 in the sample with a range for 25-hydroxyvitamin D2 expected for the replacement therapy, wherein a lower than expected amount of 25-hydroxyvitamin D2 relative to the range expected is indicative of patient non-compliance with the therapy, malabsorption of vitamin D supplements, or resistance to 25-hydroxyvitamin D2.

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