Methods for plant transformation using spectinomycin selection
First Claim
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1. A method of producing a transgenic plant comprising:
- (a) transforming at least a first soybean or cotton seed explant with a first heterologous nucleic acid sequence comprising a selectable marker conferring tolerance to spectinomycin to produce transformed cells, wherein the selectable marker gene comprises an aadA gene fused to a sequence encoding a chloroplast transit peptide; and
(b) regenerating a transgenic plant from the transformed cells, wherein the explant is contacted, prior to, concurrently with, and/or following step (a) or step (b), with at least a first media comprising spectinomycin to select transformed cells comprising said selectable marker.
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Abstract
The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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50 Claims
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1. A method of producing a transgenic plant comprising:
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(a) transforming at least a first soybean or cotton seed explant with a first heterologous nucleic acid sequence comprising a selectable marker conferring tolerance to spectinomycin to produce transformed cells, wherein the selectable marker gene comprises an aadA gene fused to a sequence encoding a chloroplast transit peptide; and (b) regenerating a transgenic plant from the transformed cells, wherein the explant is contacted, prior to, concurrently with, and/or following step (a) or step (b), with at least a first media comprising spectinomycin to select transformed cells comprising said selectable marker. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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Specification