Spatial positioning of spectrally labeled beads

• US 8,405,828 B2
• Filed: 11/17/2009
• Issued: 03/26/2013
• Est. Priority Date: 04/06/2000
• Status: Expired due to Fees

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Empedocles, Stephen, Jin, Jian, Watson, Andrew
• Primary Examiner(s): STOCK JR, GORDON J
• Application Number:
  US12/620,475
• Publication Number:
  US 20100210472A1
• Time in Patent Office: 1,225 Days
• Field of Search: None
• US Class Current: 356/326
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00432 Photolithographic masks
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00574 radioactive
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00578 electrophoric
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00657 One-dimensional arrays
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2400/0454 radiation pressure, optical tweezers
• CPC Class Codes: B01L 2400/0457 : passive flow or gravitation
• CPC Class Codes: B01L 3/5025 : for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502715 : characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502761 : specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/5085 : for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/545 : for laboratory containers
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 2015/0092 : Monitoring flocculation or agglomeration
• CPC Class Codes: G01N 2015/1472 : with colour
• CPC Class Codes: G01N 2015/1497 : Particle shape
• CPC Class Codes: G01N 2021/6441 : with two or more labels
• CPC Class Codes: G01N 2021/6482 : Sample cells, cuvettes
• CPC Class Codes: G01N 2035/00158 : Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 2035/00742 : Type of codes
• CPC Class Codes: G01N 2035/00752 : bar codes
• CPC Class Codes: G01N 21/25 : Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
• CPC Class Codes: G01N 21/253 : for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/272 : for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: G01N 21/274 : Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/278 : Constitution of standards
• CPC Class Codes: G01N 21/6428 : Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6456 : Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/6486 : Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 21/6489 : Photoluminescence of semiconductors
• CPC Class Codes: G01N 2201/04 : Batch operation; multisample devices
• CPC Class Codes: G01N 2201/06113 : Coherent sources; lasers
• CPC Class Codes: G01N 2201/12 : Circuits of general importance; Signal processing
• CPC Class Codes: G01N 33/54373 : involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.

Claims

1. A spectral label identification method, comprising:

spatially restraining, using light pressure from the restraining energy beam, a plurality of labeled bodies so as to order them into an array, each of the labeled bodies emitting a spectrum having a plurality of signals at different wavelengths;

dispersing a plurality of spectra from the spatially restrained labeled bodies simultaneously across a sensor surface, using light from an exciting energy beam; and

identifying the labeled bodies from the spectra, wherein the plurality of labeled bodies are simultaneously spatially restrained, using the light pressure from the restraining energy beam, as an array.

2. The method of claim 1, wherein the restraining energy beam and the exciting energy beam are emitted from a red laser.

3. The method of claim 1, wherein the restraining energy beam is emitted from a red laser or an infrared laser having a wavelength that does not excite the labeled bodies and the exciting energy beam is emitted from a separate laser having a wavelength that excites the labeled bodies.

4. The method of claim 1, wherein the labeled bodies are restrained within an array of openings in a multi-well plate.

5. The method of claim 4, wherein the openings in the array of openings in the multi-well plate are spaced to avoid excessive overlap of the dispersed spectra such that each of the labeled bodies can be identified from theft associated spectrum.

6. The method in claim 4, wherein the openings in the array of openings in the multi-well plate are spaced in a staggered pattern so as to increase the array opening density while avoiding excessive overlap among the dispersed spectra.

7. The method of claim 4, wherein the openings in the array of openings in the multi-well plate are deep enough to contain a set of labeled bodies.