System for flexibly representing and processing assay plates

• US 8,442,689 B2
• Filed: 03/11/2003
• Issued: 05/14/2013
• Est. Priority Date: 03/11/2002
• Status: Active Grant

• Current Assignee: Craig P. Lovell, Iii, Thomas Lucas Hampton
• Original Assignee: Iii, Thomas Lucas Hampton
• Inventors: Lovell, Craig P., Hampton, Thomas Lucas III
• Primary Examiner(s): WRIGHT, PATRICIA KATHRYN
• Application Number:
  US10/385,924
• Publication Number:
  US 20040033610A1
• Time in Patent Office: 3,717 Days
• Field of Search: None
• US Class Current: 700/266
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/545 for laboratory containers
• CPC Class Codes: B01L 9/50 Clamping means, tongs in general F16B2/06
• CPC Class Codes: B01L 9/56 Means for indicating position of a recipient or sample in an array
• CPC Class Codes: G01N 2035/00831 identification of the sample, e.g. patient identity, place of sampling
• CPC Class Codes: G01N 2035/0491 Position sensing, encoding; closed-loop control
• CPC Class Codes: G01N 35/00584 Control arrangements for automatic analysers
• CPC Class Codes: G01N 35/00871 Communications between instruments or with remote terminals
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: G05B 15/02 electric
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

A flexible instrument control and data storage/management system and method for representing and processing assay plates having one or more predefined plate locations is disclosed. The system utilizes a graph data structure, layer objects and data objects. The layer objects map the graph data structure to the data objects. The graph data structure can comprise one node for each of the one or more predefined plate locations, wherein the nodes can be hierarchically defined according to a predefined plate location hierarchy. Each node can be given a unique node identifier, a node type and a node association that implements the predefined plate location hierarchy. The layer objects can include an index that maps the node identifiers to the data objects.

Claims

1. A flexible instrument control and data storage/management system for representing and processing an assay plate having one or more predefined plate locations, the system comprising a non-transitory computer readable medium device including a software architecture that performs the steps comprising:

(a) accessing a database comprising a plate type directory including a plurality of graph data structures representing the physical structure of said assay plate, wherein said graph data structures each comprise a node for each of the one or more predefined plate locations, and said node is hierarchically arranged to correspond to the physical structure of the assay plate; and

(b) accessing at least one layer object that maps at least one data object to one or more nodes within a particular graph data structure, wherein said at least one data object comprises one or more data records,

wherein the at least one data object is not stored on said database comprising the graph data structure and wherein the assay plate has a predefined number of wells arranged in a plurality of well groupings, and wherein the one or more predefined plate locations include;

one or more spots in each well; and

one or more sectors associated with each grouping of wells, wherein the one or more sectors each contain the one or more spots, respectively, from each well of the associated group of wells.

2. The system according to claim 1, each node having a unique node identifier and a node type.

3. The system according to claim 2, wherein the node type includes one of a plate, sector, well or spot.

4. The system according to claim 3, each of the at least one layer objects having an index, wherein the index maps each node identifier to one or more of the at least one data objects.

5. The system according to claim 3, wherein the graph data structure is a tree data structure.

6. The system according to claim 1 wherein the assay plate comprises 96 wells arranged in a grid of 8 rows and 12 columns of wells, wherein each well grouping is one column of wells.

7. The system according to claim 1, wherein the assay plate has associated geometry data that includes one or more predefined points of interest, the system further comprising:

a tree of one or more multi-dimensional coordinate space objects having a plurality of subspaces, the subspaces each being anchored in the coordinate space object at a predefined subspace anchor point, wherein one or more of the plurality of subspaces may contain one or more of the predefined points of interest.

8. The system of claim 1, wherein the hierarchical containment relationships include three or more hierarchies of containment.

9. The system according to claim 1 wherein said computer readable medium comprises said plate type directory comprising said graph data structure.

10. A non-transitory computer readable medium having stored thereon a computer program which, when executed by a computer system operatively connected to an assay system, causes the assay system to perform a method of representing and processing an assay plate having one or more predefined plate locations, the method comprising the steps of:

(a) accessing a database comprising a plate type directory including a plurality of graph data structures representing the physical structure of the assay plate, wherein said graph data structures each comprise a node for each of the one or more predefined plate locations, and said node is hierarchically arranged to correspond to the physical structure of the assay plate; and

(b) accessing at least one layer object that maps at least one data object to one or more nodes within a particular graph data structure, wherein the at least one data object comprises one or more data records,

wherein the at least one data object is not stored on said database comprising the graph data structure, wherein the one or more predefined plate locations include;

one or more spots in each well; and

one or more sectors associated with each grouping of wells, wherein the one or more sectors each contain the one or more spots, respectively, from each well of the associated group of wells.

11. The computer readable medium according to claim 10, wherein each node has a unique node identifier and a node type.

12. The computer readable medium according to claim 11, wherein the node type includes a plate, sector, well, or spot.

13. The computer readable medium according to claim 12, wherein each of the at least one layer objects having an index, wherein the index maps each node identifier to one or more of the at least one data objects.

14. The computer readable medium according to claim 12, wherein the graph data structure is a tree data structure.

15. The computer readable medium according to claim 10 wherein the assay plate comprises 96 wells arranged in a grid of 8 rows and 12 columns of wells, wherein each well grouping is one column of wells.

16. The computer readable medium according to claim 10, wherein the assay plate has associated geometry data that includes one or more predefined points of interest, the system further comprising:

a tree of one or more multi-dimensional coordinate space objects having a plurality of subspaces, the subspaces each being anchored in the coordinate space object at a predefined subspace anchor point, wherein one or more of the plurality of subspaces may contain one or more of the predefined points of interest.

17. The computer readable medium according to claim 10, wherein the hierarchical containment relationships include three or more hierarchies of containment.

18. The computer readable medium according to claim 10 wherein said computer readable medium comprises said plate type directory comprising said graph data structure.