Redox polymers for use in analyte monitoring

• US 8,444,834 B2
• Filed: 03/19/2008
• Issued: 05/21/2013
• Est. Priority Date: 11/15/1999
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Liu, Zenghe, Feldman, Benjamin J., Mao, Fei, Heller, Adam
• Primary Examiner(s): Van, Luan
• Assistant Examiner(s): Kessel, Maris R
• Application Number:
  US12/051,835
• Publication Number:
  US 20120132525A1
• Time in Patent Office: 1,889 Days
• Field of Search: 204/403.04, 204/403.1, 204/403.14, 526/161, 546/2, 548/101
• US Class Current: 204/403.14
• CPC Class Codes: C07F 15/0026 without a metal-carbon linkage
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/004 mediator-assisted

Abstract

Polymers for use as redox mediators in electrochemical biosensors are described. The transition metal complexes attached to polymeric backbones can be used as redox mediators in enzyme based electrochemical sensors. In such instances, transition metal complexes accept electrons from, or transfer electrons to, enzymes at a high rate and also exchange electrons rapidly with the sensor. The transition metal complexes include at least one substituted or unsubstituted biimidazole ligand and may further include a second substituted or unsubstituted biimidazole ligand or a substituted or unsubstituted bipyridine or pyridylimidazole ligand.

Claims

1. A redox polymer comprising

(a) a polymeric backbone;

(b) a cross-linker; and

(c) a transition metal complex having the following formula;

2. The redox polymer of claim 1, wherein M is osmium and the transition complex has the following formula:

3. The redox polymer of claim 2, wherein at least one of R₁, R₂, and R′

₁ comprises a reactive group selected from the group consisting of carboxy, activated ester, sulfonyl halide, sulfonate ester, isocyanate, isothiocyanate, epoxide, aziridine, halide, aldehyde, ketone, amine, acrylamide, thiol, acyl azide, acyl halide, hydrazine, hydroxyamine, alkyl halide, imidazole, pyridine, phenol, alkyl sulfonate, halotriazine, imido ester, maleimide, hydrazide, hydroxy, and photo-reactive azido aryl groups.

4. The redox polymer of claim 3, wherein at least one of R₁, R₂, and R′

₁ is coupled to a polymeric backbone.

5. The redox polymer of claim 1, wherein the cross-linker is PEGDGE.

6. The redox polymer of claim 1, having the following structure:

7. The redox polymer of claim 1, wherein the polymeric backbone is a poly(vinylpyridine) having the structure: