Transient decay amperometry
First Claim
1. A method for determining an analyte concentration in a sample, comprising:
- reacting an analyte in a sample with an ionizing agent;
forming a measurable species from the reaction between the sample and the ionizing agent during an incubation period from 0.1 to 8 seconds, where the measurable species concentration in the sample is responsive to an analyte concentration in the sample;
applying an input signal to the sample after the incubation period, where the input signal includes excitations and relaxations, whereat least two of the excitations have a duration from 0.1 to 5 seconds, and whereat least one of the relaxations has a duration from 0.4 to 1 second;
generating an output signal from the excitation following the at least one of the relaxations having the duration from 0.4 to 1 second, the output signal having a transient decay responsive to a redox reaction of the measurable species; and
determining the analyte concentration of the sample from the transient decay of the generated output signal.
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Accused Products
Abstract
A biosensor system determines an analyte concentration of a biological sample using an electrochemical process without Cottrell decay. The biosensor system generates an output signal having a transient decay, where the output signal is not inversely proportional to the square root of the time. The transient decay is greater or less than the −0.5 decay constant of a Cottrell decay. The transient decay may result from a relatively short incubation period, relatively small sample reservoir volumes, relatively small distances between electrode surfaces and the lid of the sensor strip, and/or relatively short excitations in relation to the average initial thickness of the reagent layer. The biosensor system determines the analyte concentration from the output signal having a transient decay.
240 Citations
50 Claims
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1. A method for determining an analyte concentration in a sample, comprising:
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reacting an analyte in a sample with an ionizing agent; forming a measurable species from the reaction between the sample and the ionizing agent during an incubation period from 0.1 to 8 seconds, where the measurable species concentration in the sample is responsive to an analyte concentration in the sample; applying an input signal to the sample after the incubation period, where the input signal includes excitations and relaxations, where at least two of the excitations have a duration from 0.1 to 5 seconds, and where at least one of the relaxations has a duration from 0.4 to 1 second; generating an output signal from the excitation following the at least one of the relaxations having the duration from 0.4 to 1 second, the output signal having a transient decay responsive to a redox reaction of the measurable species; and determining the analyte concentration of the sample from the transient decay of the generated output signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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33. A method for determining an analyte concentration in a sample, comprising:
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reacting an analyte in a sample with an ionizing agent; forming a measurable species from the reaction between the sample and the ionizing agent during an incubation period of at most 8 seconds, where the measurable species concentration in the sample is responsive to an analyte concentration in the sample; applying an input signal to the sample after the incubation period, where the input signal includes excitations and relaxations, where at least one of the excitations has a duration from 0.1 to 1 second, and where at least one of the relaxations has a duration from 0.1 to 3 seconds; generating an output signal from the at least one of the excitations having the duration from 0.1 to 1 second, the output signal having a transient decay responsive to a redox reaction of the measurable species; and determining the analyte concentration of the sample from the transient decay of the generated output signal. - View Dependent Claims (36, 37)
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34. A method for determining an analyte concentration in a sample, comprising:
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reacting an analyte in a sample with an ionizing agent; forming a measurable species from a redox reaction between a portion of the analyte in the sample and the ionizing agent during an incubation period from 0.1 to 8 seconds; applying an input signal to the sample after the incubation period; generating an output signal responsive to the analyte concentration in the sample from the input signal, the output signal having a transient decay responsive to the measurable species formed from the portion of the analyte in the sample; and determining the analyte concentration of the sample from the transient decay of the output signal within 3 seconds of applying the input signal to the sample.
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35. A method for determining an analyte concentration in a sample, comprising:
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reacting an analyte in a sample with an ionizing agent; forming a measurable species from a redox reaction between the analyte in the sample and the ionizing agent during an incubation period of from 0.1 to 8 seconds; applying an input signal to the sample after the incubation period, the input signal including excitations and relaxations, where at least two of the excitations have a duration from 0.1 to 5 seconds and at least two of the relaxations have a duration from 0.1 to 3 seconds; generating an output signal responsive to the analyte concentration in the sample from at least one of the excitations within 0.5 to 5 seconds of reacting the analyte in the sample with the ionizing agent, the output signal having a transient decay of continually decreasing currents responsive to the measurable species; and determining the analyte concentration of the sample from the continually decreasing current decay of the output signal. - View Dependent Claims (38, 39)
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Specification