Systems and methods for membraneless dialysis

• US 8,491,516 B2
• Filed: 11/17/2011
• Issued: 07/23/2013
• Est. Priority Date: 03/14/2003
• Status: Expired due to Fees

• Current Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Original Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Inventors: Leonard, Edward F., West, Alan C., Shapley, Nina C., Tang, Zhongliang
• Primary Examiner(s): MARCETICH, ADAM M
• Application Number:
  US13/298,344
• Publication Number:
  US 20120061304A1
• Time in Patent Office: 614 Days
• Field of Search: 604/4.01, 604/5.01, 604/5.04, 604/6.04, 604/6.09, 604/28, 422/44
• US Class Current: 604/5.04
• CPC Class Codes: A61M 1/14 Dialysis systems; Artificial kidneys; Blood oxygenators semi-permeable membranes characterised by the material, manufacturing processes therefor B01D71/00 ; Reciprocating systems for treatment of body fluids, e.g. single needle systems for hemofiltration or pheresis
• CPC Class Codes: A61M 2206/11 Laminar flow
• CPC Class Codes: B01D 11/0288 Applications, solvents
• CPC Class Codes: B01D 11/0492 Applications, solvents used
• CPC Class Codes: B01D 11/0496 by extraction in microfluidic devices
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00

Abstract

Devices, systems and methods are disclosed for removing undesirable materials from a sample fluid by contact with a second fluid. The sample fluid flows as a thin layer adjacent to, or between, concurrently flowing layers of the second fluid, without an intervening membrane. In various embodiments, a secondary separator is used to restrict the removal of desirable substances and effect the removal of undesirable substances from blood. The embodiments may be used for the removal of components from a sample fluid that vary in size. When blood is the sample fluid, for example, this may include the removal of small molecules, middle molecules, macromolecules, macromolecular aggregates, and cells, from the blood sample to the extractor fluid.

Claims

1. A method of performing a blood treatment, comprising:

withdrawing blood from a live patient and flowing withdrawn blood into a stack of rectangular microfluidic channels each sized and shaped, and at a flow rate, to produce a shear rate such that cellular components of the blood migrate to the center of each microfluidic channel and leave a sheath as cell-free, essentially pure plasma;

each microfluidic channel having an aspect ratio perpendicular to the flow direction of at least 10, the minimum dimension being a depth and the maximum dimension being a width;

withdrawing the essentially pure plasma from each microfluidic channel through two outlets positioned across the depth;

simultaneously withdrawing plasma-depleted blood from each microfluidic channel from the middle of each microfluidic channel between the two outlets;

combining withdrawn pure plasma flow from the stack of microfluidic channels and pumping the withdrawn pure plasma at a positive pressure through a membrane filter to ultrafilter water and uremic toxins therefrom to produce dehydrated plasma at an outlet of the filter;

returning the dehydrated plasma and the plasma-depleted blood to the patient;

performing the above operations continuously over a treatment period of time encompassing a majority of a day, the stack being a component of a wearable system,

wherein the rate of water removal over the treatment period of time is sufficient to regulate the water burden of a patient with renal failure.

2. The method of claim 1, wherein the returning the dehydrated plasma to the patient includes pumping the dehydrated plasma into the stack of microfluidic channels.

3. The method of claim 2, wherein the returning the dehydrated plasma to the patient includes shunting the dehydrated plasma directly into the blood stream of the patient.

4. The method of claim 2, wherein the pumping the withdrawn pure plasma at a positive pressure through a membrane filter to ultrafilter water and uremic toxins from the pure plasma includes diafiltering the pure plasma.

5. The method of claim 1, wherein the returning the dehydrated plasma to the patient includes shunting the dehydrated plasma directly into the blood stream of the patient.

6. The method of claim 5, wherein the pumping the withdrawn pure plasma at a positive pressure through a membrane filter to ultrafilter water and uremic toxins from the pure plasma includes diafiltering the pure plasma.

7. The method of claim 1, wherein the pumping the withdrawn pure plasma at a positive pressure through a membrane filter to ultrafilter water and uremic toxins from the pure plasma includes diafiltering the pure plasma.

8. The method of claim 1, further comprising regulating the flow through the microfluidic channels to decrease the flow of blood in response to a detection of a threshold amount of blood cells in the plasma withdrawn from each microfluidic channel through said two outlets.

9. The method of claim 1, wherein the aspect ratio is at least 50.

10. The method of claim 1, wherein the channel depth is less than 100 microns.

11. The method of claim 1, wherein the ultrafiltering of water and uremic toxins from the pure plasma retains all the proteins in the dehydrated plasma.