Electrochemical labels derived from siderophores

• US 8,492,158 B2
• Filed: 03/31/2008
• Issued: 07/23/2013
• Est. Priority Date: 03/31/2008
• Status: Active Grant

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Eason, Robert G.
• Primary Examiner(s): Tsang, Cecilia J
• Assistant Examiner(s): NIEBAUER, RONALD T
• Application Number:
  US12/059,744
• Publication Number:
  US 20090246763A1
• Time in Patent Office: 1,940 Days
• Field of Search: None
• US Class Current: 436/84
• CPC Class Codes: C07H 15/26 Acyclic or carbocyclic radicals, substituted by hetero rings
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2563/137 Metal/ion, e.g. metal label

Abstract

Disclosed are tri-nuclear metal complexes and salts thereof, such as tri-nuclear osmium or ruthenium complexes or salts thereof, suitable for use as electrochemical labels. Also disclosed are oligonucleotide probes with an attached electrochemical label, methods of nucleic acid amplification, methods of sequencing, and kits for nucleic acid amplification and sequencing having oligonucleotide probes including an electrochemical label. The electrochemical labels are synthesized from siderophores.

Claims

1. The tri-nuclear metal complex comprising structure (I′

), or a salt thereof;

2. The tri-nuclear metal complex of claim 1, wherein the tri-nuclear complex has an E_1/2 value of from about −

200 to about 500 mV vs. Ag/AgCl.

3. The tri-nuclear metal complex of claim 1, wherein the counterion is selected from the group consisting of PF₆⁻

, TFA⁻

, OAc⁻

, Cl⁻

and Br⁻

.