High level expression of recombinant toxin proteins

  • US 8,530,171 B2
  • Filed: 03/28/2011
  • Issued: 09/10/2013
  • Est. Priority Date: 03/30/2010
  • Status: Active Grant
First Claim
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1. A method for producing a recombinant toxin protein in a Pseudomonad host cell, said method comprising:

  • ligating into an expression vector a nucleotide sequence encoding the toxin protein;

    transforming the Pseudomonad host cell with the expression vector; and

    culturing the transformed Pseudomonad host cell in a culture media suitable for the expression of the recombinant toxin protein;

    wherein the recombinant toxin protein is CRM 197, and wherein the recombinant protein is produced at a yield of soluble or active CRM197 protein of about 0.2 grams per liter to about 12 grams per liter.

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