Polynucleotides encoding a NAX2 polypeptide and methods for enhancing salinity tolerance in plants

• US 8,536,429 B2
• Filed: 07/12/2007
• Issued: 09/17/2013
• Est. Priority Date: 07/12/2006
• Status: Active Grant

• Current Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Original Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Inventors: Platten, John Damien, Byrt, Caitlin, Spielmeyer, Wolfgang, Lagudah, Evans, James, Richard Alexander, Munns, Rana Ellen
• Primary Examiner(s): Worley, Cathy Kingdon
• Assistant Examiner(s): BURAN, ASHLEY KATE
• Application Number:
  US12/309,157
• Publication Number:
  US 20100313289A1
• Time in Patent Office: 2,259 Days
• Field of Search: None
• US Class Current: 800/320.3
• CPC Class Codes: A01H 5/10 Seeds
• CPC Class Codes: A01H 6/4678 Triticum sp. [wheat]
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8273 for drought, cold, salt resistance

Abstract

The present invention relates to polypeptides, and polynucleotides encoding therefor, with cation transporter activity. In particular, the present invention relates to methods for producing, identifying, and/or breeding transgenic or non-transgenic plants, especially wheat or barley plants, with enhanced tolerance to saline and/or sodic soils, and/or reduced sodium accumulation in an aerial part of the plant. Also provided are plants produced using these methods.

Claims

1. A wheat plant other than a Triticum monococcum wheat plant which is homozygous for a Nax2 gene that encodes a Nax2 polypeptide comprising amino acids whose sequence is at least 95% identical to the sequence set forth in SEQ ID NO:

1 and confers enhanced tolerance to saline and/or sodic soils, and/or reduced sodium accumulation in an aerial part of the wheat plant in comparison to a wheat plant that is not homozygous for the Nax2 gene.

2. The wheat plant of claim 1 which is durum wheat.

3. The wheat plant of claim 2 which has a genetic background comprising less than 50% of the genetic background of durum Line 149, durum Line 5049, or of the cultivar Tamaroi.

4. The wheat plant of claim 1 which is hexaploid wheat.

5. The wheat plant of claim 1, wherein the gene is on chromosome 5A or chromosome 4A.

6. The wheat plant of claim 1, wherein the gene encodes a polypeptide which comprises a cysteine residue at a position corresponding to amino acid number 232 of SEQ ID NO:

1 and/or an aspartic acid residue at a position corresponding to amino acid number 294 of SEQ ID NO;

1.

7. The wheat plant of claim 1 which further comprises an allele of a Nax1 gene, and/or an allele of a Kna1 gene, which confers enhanced tolerance to saline and/or sodic soils, and/or reduced sodium accumulation in an aerial part of the wheat plant in comparison to a wheat plant that is not homozygous for the Nax2 gene nor comprises a Nax1 and/or Kna1 gene.

8. The wheat plant of claim 7 which is characterized by a sheath:

blade ratio of Na⁺ concentration in leaf 3 of at least 1.5 when grown for at least 10 days in a hydroponic system using a growth medium having a NaCl concentration of 50 mM.

9. The wheat plant of claim 1, wherein the gene encodes a polypeptide that transports sodium across a cell membrane and out of the xylem of a plant to a greater extent than it transports potassium.

14. A process of producing wheat grain, the process comprising;

i) growing a wheat plant of claim 1, and

ii) harvesting the wheat grain from the wheat plant.

15. Wheat grain other than Triticum monococcum wheat grain produced by the process of claim 14, wherein the wheat grain is homozygous for a Nax2 gene that encodes a Nax2 polypeptide comprising an amino acids whose sequence is at least 95% identical to the sequence set forth as SEQ ID NO:

1 and confers enhanced tolerance to saline and/or sodic soils, and/or reduced sodium accumulation in an aerial part of a wheat plant in comparison to a wheat plant that is not homozygous for the Nax2 gene.

16. A process of producing flour, wholemeal, starch or other product obtained from wheat grain, the process comprising;

i) obtaining wheat grain of claim 15, and

ii) extracting the flour, wholemeal, starch or other product from the wheat grain.

17. A process for making a food product, the process comprising processing the wheat grain produced by the process of claim 14 with another ingredient so as to make the food product.

10. A method of obtaining a wheat plant, the method comprising;

i) crossing two wheat plants of the same species of which at least one wheat plant comprises a Nax2 locus comprising an allele that encodes a Nax2 polypeptide comprising amino acids whose sequence is at least 95% identical to the sequence set forth as SEQ ID NO;

1 and confers enhanced tolerance to saline and/or sodic soils, and/or which confers reduced sodium accumulation in an aerial part of the wheat plant, and

ii) screening progeny wheat plants from the cross for the presence or absence of said Nax2 locus by detecting a nucleic acid molecule of the wheat plant, wherein the nucleic acid molecule is linked to, and/or comprises at least a part of, said Nax2 locus comprising the allele that confers enhanced tolerance to saline and/or sodic soils, and/or that confers reduced sodium accumulation in an aerial part of the wheat plant,

wherein progeny with said allele have enhanced tolerance to saline and/or sodic soils, and/or have reduced sodium accumulation in an aerial part of the wheat plant, when compared to progeny lacking said allele.

11. The method of claim 10, wherein the wheat plants of the same species of step i) are hexaploid wheat plants.

12. The method of claim 11, wherein the cross is between a durum wheat plant comprising said allele and a hexaploid wheat plant lacking said allele.

13. The method of claim 10, wherein step i) comprises crossing a first parent wheat plant with a second parent wheat plant, wherein the second wheat plant comprises a Nax2 locus which comprises the allele, and wherein the method further comprises backcrossing the progeny of the cross of step i) with wheat plants of the same genotype as the first parent wheat plant for a sufficient number of times to produce a wheat plant with a majority of the genotype of the first parent but comprising said allele.