Compositions comprising alternating 2′-modified nucleosides for use in gene modulation
First Claim
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1. A composition comprising first and second chemically synthesized oligomeric compounds, wherein:
- the first oligomeric compound is fully complementary to and capable of hybridizing with said second oligomeric compound and to a selected nucleic acid target;
at least one of said first and second oligomeric compounds comprises a contiguous sequence of linked nucleosides wherein the sequence defines an alternating motif having the formula;
5′
-Q(-L-Z-L-Q)n(-L-Z)nn-3′
wherein;
each L is an internucleoside linking group;
each Q is a nucleoside having a 2′
-F substituent group and each Z is a β
-D-deoxyribonucleoside;
oreach Q is β
-D-deoxyribonucleoside and each Z is a nucleoside having a 2′
-F substituent group;
n is from about 8 to about 14 and nn is 0 or 1; and
each of said oligomeric compounds is from 12 to 30 linked nucleosides in length.
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Abstract
The present invention provides compositions comprising at least one oligomeric compound comprising an alternating motif and further include a region that is complementary to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In preferred embodiments the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression.
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24 Claims
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1. A composition comprising first and second chemically synthesized oligomeric compounds, wherein:
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the first oligomeric compound is fully complementary to and capable of hybridizing with said second oligomeric compound and to a selected nucleic acid target; at least one of said first and second oligomeric compounds comprises a contiguous sequence of linked nucleosides wherein the sequence defines an alternating motif having the formula;
5′
-Q(-L-Z-L-Q)n(-L-Z)nn-3′wherein; each L is an internucleoside linking group; each Q is a nucleoside having a 2′
-F substituent group and each Z is a β
-D-deoxyribonucleoside;
oreach Q is β
-D-deoxyribonucleoside and each Z is a nucleoside having a 2′
-F substituent group;n is from about 8 to about 14 and nn is 0 or 1; and each of said oligomeric compounds is from 12 to 30 linked nucleosides in length. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification