Detection of methylated DNA and DNA mutations

• US 8,609,333 B2
• Filed: 10/01/2008
• Issued: 12/17/2013
• Est. Priority Date: 10/01/2007
• Status: Active Grant

• Current Assignee: University of Southern California
• Original Assignee: University of Southern California
• Inventors: Zhou, Chongwu, Thompson, Mark E., Yang, Allen S., Cote, Richard James
• Primary Examiner(s): Martinell, James
• Application Number:
  US12/680,806
• Publication Number:
  US 20100292348A1
• Time in Patent Office: 1,903 Days
• Field of Search: None
• US Class Current: 435/6.1
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2522/10 Nucleic acid binding proteins
• CPC Class Codes: C12Q 2522/101 Single or double stranded nucleic acid binding proteins
• CPC Class Codes: C12Q 2523/125 Bisulfite(s)
• CPC Class Codes: C12Q 2537/164 Methylation detection other then bisulfite or methylation sensitive restriction endonucleases
• CPC Class Codes: C12Q 2563/155 Particles of a defined size, e.g. nanoparticles
• CPC Class Codes: C12Q 2565/519 characterised by the capture moiety being a single stranded oligonucleotide
• CPC Class Codes: C12Q 2565/607 being a sensor, e.g. electrode
• CPC Class Codes: C12Q 2565/631 being a biochannel or pore
• CPC Class Codes: C12Q 2600/154 Methylation markers
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: G01N 27/4146 involving nanosized elements, e.g. nanotubes, nanowires
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The present invention relates to various methods of detecting DNA methylation and defected DNA. In one embodiment, the invention provides a nanosensor bound to a probe that is complementary to a DNA methylation sequence.

Claims

1. A method of preparing a nanosensor to detect DNA methylation, comprising:

providing a nanosensor; and

attaching a probe to the nanosensor,

wherein the probe is adapted to bind a methylated DNA sequence and an agent,

wherein the nanosensor is configured to detectably change in conductance when the methylated DNA sequence is itself bound by the agent.

2. The method of claim 1, wherein the nanosensor comprises nanotube and nanowire surfaces.

3. The method of claim 1, wherein the nanosensor is an NW/NT sensor.

4. The method of claim 1, wherein the probe comprises a polynucleotide.

5. The method of claim 1, wherein the probe is adapted to bind an agent.

6. The method of claim 5, wherein the agent comprises a signal amplifier.

7. The method of claim 1, wherein the probe comprises SEQ. ID. NO.:

1, SEQ. ID. NO.;

2, SEQ. ID. NO.;

3, SEQ. ID. NO.;

4, or a combination thereof.

8. The method of claim 1, wherein the probe comprises SEQ. ID. NO.:

5, SEQ. ID. NO.;

6, SEQ. ID. NO.;

7, SEQ. ID. NO.;

8, SEQ. ID. NO.;

9, SEQ. ID. NO.;

10, SEQ. ID. NO.;

11, SEQ. ID. NO.;

12, SEQ. ID. NO.;

13, or a combination thereof.