Number coding for identification of subtypes of coded types of solid phase carriers
First Claim
1. A method of employing numerical encoding of particles in a bioassay, comprising:
- providing uniquely color encoded groups of particles having ligands attached to said particles wherein each group of particles comprises two or more subtypes of particles having different ligands attached to said two or more subtypes of particles;
wherein the number of each subtype of particles provided has a unique decomposition into one or more summands, such that no partial sum of one or more summands can be obtained in any other way of combining summands, and no summand is itself the sum of two or more of the other summands;
conducting an assay by contacting the groups of particles with a sample comprising analytes;
determining, for each group of particles, the number of particles generating a positive assay signal, indicating reaction between a ligand and an analyte;
comparing, for each group of particles, said number of particles generating a positive assay signal with the number of particles of each subtype; and
determining, for each group of particles, the analytes present in the sample based on said comparing step.
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Accused Products
Abstract
Disclosed is number coding of pairs (“doublets”) or small sets (“multiplets”) of solid phase carriers which provides distinguishable subtypes of a given type of such carriers, where each carrier type is distinguishable on the bases of a different code. Such number coding is useful for augmenting a coding system, such as a color code, and thereby effectively multiplying the number of “colors” (distinguishable sub-types). It can be applied, for example, to determine whether a sample is homozygous or heterozygous at a number of different sites for one of two different alleles, where the same color code is applied for each of the two alleles, and the alleles with the same color code are distinguished by knowing how many carriers are associated with molecules which detect each different allele.
547 Citations
8 Claims
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1. A method of employing numerical encoding of particles in a bioassay, comprising:
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providing uniquely color encoded groups of particles having ligands attached to said particles wherein each group of particles comprises two or more subtypes of particles having different ligands attached to said two or more subtypes of particles; wherein the number of each subtype of particles provided has a unique decomposition into one or more summands, such that no partial sum of one or more summands can be obtained in any other way of combining summands, and no summand is itself the sum of two or more of the other summands; conducting an assay by contacting the groups of particles with a sample comprising analytes; determining, for each group of particles, the number of particles generating a positive assay signal, indicating reaction between a ligand and an analyte; comparing, for each group of particles, said number of particles generating a positive assay signal with the number of particles of each subtype; and determining, for each group of particles, the analytes present in the sample based on said comparing step. - View Dependent Claims (2, 3)
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4. A method of employing numerical coding of particles, comprising:
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providing uniquely color encoded groups of particles having peptides or antibodies attached to said particles, wherein each group of particles comprises two or more subtypes of particles having different peptides or antibodies attached to said two or more subtypes of particles; wherein the number of each subtype of particles provided has a unique decomposition into one or more summands, such that no partial sum of one or more summands can be obtained in any other way of combining summands, and no summand is itself the sum of two or more of the other summands; conducting an assay by contacting the groups of particles with a sample comprising analytes; determining for each group of particles, the number of particles generating a positive assay signal, indicating reaction between a peptide or antibody and an analyte; comparing for each group of particles, said number of particles generating a positive assay signal with the number of particles of each subtype; and determining for each group of particles, the analytes present in the sample based on said comparing step. - View Dependent Claims (5, 6, 7, 8)
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Specification