Use of plants with reduced levels of de-esterified homogalacturonan in the cell wall or portions thereof for improving the saccharification of plant biomasses

• US 8,637,734 B2
• Filed: 12/29/2009
• Issued: 01/28/2014
• Est. Priority Date: 12/30/2008
• Status: Active Grant

• Current Assignee: Universita' Degli Studi Di Roma La Sapienza (Repubblica Italiana)
• Original Assignee: Universita' Degli Studi Di Roma La Sapienza (Repubblica Italiana)
• Inventors: Cervone, Felice, De Lorenzo, Giulia, Salvi, Giovanni, Bellincampi, Daniela, Ferrari, Simone, Lionetti, Vincenzo, Francocci, Fedra, Pontiggia, Daniela
• Primary Examiner(s): Page, Brent T
• Application Number:
  US12/648,644
• Publication Number:
  US 20100170008A1
• Time in Patent Office: 1,491 Days
• Field of Search: None
• US Class Current: 800/284
• CPC Class Codes: C12N 15/8246 Non-starch polysaccharides, e.g. cellulose, fructans, levans

Abstract

The present invention relates to expression in plants of pectinolytic enzymes and of pectin methylesterase inhibitors for increasing the degradability of plant tissues through enzymatic digestion, thus improving saccharification efficiency.

Claims

1. A method of producing sugars from plant biomass, the method comprising enzymatically hydrolyzing biomass of transgenic plants having a reduced content of de-esterified homogalacturonan (HGA) in the pectins of said plants cell walls and a reduced resistance to saccharification with respect to control plants, wherein said transgenic plants are obtained by transformation with a gene encoding an inhibitor of pectin methyl esterases, wherein the inhibitor of pectin methyl esterases has the amino acid sequence of SEQ ID NO:

4.

2. The method according to claim 1 wherein the gene encoding an inhibitor of pectin methyl esterases has the nucleotide sequence of SEQ ID NO:

3.

3. The method of claim 1 wherein the reduced resistance to saccharification with respect to control plants provides an enzymatic hydrolyzation efficiency expressed as percentage of total sugars released, of at least 55%, compared to an enzymatic hydrolysis efficiency of about 20% for plant biomass from non transformed plants, at 24 hours.

4. The method of claim 1 further comprising a step of pretreating the biomass of transgenic plants with a dilute acid.