Gene expression control in plants

• US 8,669,108 B2
• Filed: 03/30/2009
• Issued: 03/11/2014
• Est. Priority Date: 04/03/2008
• Status: Expired due to Fees

• Current Assignee: insight genomics limited
• Original Assignee: Vialactia Biosciences (Nz) Limited
• Inventors: Puthigae, Sathish, Phillips, Jonathan Robert, Withana, Nimali Piyushika, Smith-Espinoza, Claudia Jeannette, Bryant, Catherine Jane, Templeton, Kerry Robert, Bajaj, Shivendra
• Primary Examiner(s): Zhou, Joe
• Assistant Examiner(s): BOGGS, RUSSELL T
• Application Number:
  US12/935,952
• Publication Number:
  US 20110179517A1
• Time in Patent Office: 1,807 Days
• Field of Search: None
• US Class Current: 435/419
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8216 Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Abstract

The invention provides the isolated promoter polynucleotide sequence of SEQ ID NO:1, and fragments and variants thereof. The invention also provides constructs, plant cells and plants genetically modified to contain the promoter polynucleotide. The invention also provides methods for producing plants with altered gene expression and traits via genetic transformation of plants with the promoter polynucleotides.

Claims

1. A host cell, plant cell or plant transformed with a promoter polynucleotide, wherein the promoter polynucleotide comprises at least one of:

a) the sequence of SEQ ID NO;

1; and

b) a sequence with at least 95% identity to the sequence of SEQ ID NO;

1.

2. The host cell, plant cell or plant of claim 1, wherein the promoter polynucleotide is capable of controlling transcription of an operably linked polynucleotide in a plant.

3. The host cell, plant cell or plant of claim 1, in which the promoter polynucleotide is capable of controlling transcription of an operably linked polynucleotide sequence in at least one of leaves, internodes, roots and flowers of a plant.

4. The host cell, plant cell or plant of claim 1, in which the promoter polynucleotide is capable of driving transcription of an operably linked polynucleotide sequence more abundantly in above-ground plant parts than in the below-ground plant parts.

5. The host cell, plant cell or plant of claim 1, in which the promoter polynucleotide is capable of driving transcription of an operably linked polynucleotide sequence more abundantly in leaves and internodes than in roots and flowers of a plant.

6. The host cell, plant cell or plant of claim 1, wherein the promoter polynucleotide is part of a genetic construct.

7. The host cell, plant cell or plant of claim 6, wherein the promoter polynucleotide is operably linked to a polynucleotide sequence to be expressed.

15. A seed, propagule, progeny, part or product of a plant of claim 1.

16. The seed, propagule, progeny, part or product of claim 15, that contains a polynucleotide comprising at least one of:

a) the sequence of SEQ ID NO;

1; and

b) a sequence with at least 95% identity to the sequence of SEQ ID NO;

1.

8. A method for modifying expression of at least one polynucleotide in a plant cell or plant, the method comprising transformation of the plant cell or plant with a promoter polynucleotide of comprising at least one of:

a) the sequence of SEQ ID NO;

1; and

b) a sequence with at least 95% identity to the sequence of SEQ ID NO;

1.

9. The method of claim 8 wherein the promoter polynucleotide is part of a genetic construct.

10. A method for producing a plant cell or plant with modified expression of at least one polynucleotide, the method comprising:

(a) transforming the plant cell or plant with a promoter polynucleotide comprising at least one of;

i) the sequence of SEQ ID NO;

1; and

ii) a sequence with at least 95% identity to the sequence of SEQ ID NO;

1;

(b) cultivating the transgenic plant cell or plant under conditions conducive for the promoter polynucleotide to drive transcription.

11. The method of claim 10, wherein the promoter polynucleotide is part of a genetic construct.

12. The method of claim 10, wherein the promoter polynucleotide is part of a genetic construct in which the promoter polynucleotide is operably linked to a polynucleotide sequence to be expressed.

13. The method of claim 10 which results in a plant cell or plant with a modified phenotype.

14. A plant cell or plant produced by the method of claim 10.