Calibratable sensor unit for reaction vessels

• US 8,673,624 B2
• Filed: 02/10/2011
• Issued: 03/18/2014
• Est. Priority Date: 02/10/2010
• Status: Active Grant

• Current Assignee: Hamilton Bonaduz AG (Hamilton Company)
• Original Assignee: Hamilton Bonaduz AG (Hamilton Company)
• Inventors: Loebbert, Andreas, Schoenfuss, Dirk
• Primary Examiner(s): BOWERS, NATHAN ANDREW
• Application Number:
  US13/024,662
• Publication Number:
  US 20110236962A1
• Time in Patent Office: 1,132 Days
• Field of Search: 435/287.1, 435/287.2, 435/289.1, 73/10.1, 73/10.2, 73/10.3
• US Class Current: 435/287.1
• CPC Class Codes: C12M 23/26 flexible flexible containers for laboratory use B01L3/505
• CPC Class Codes: C12M 23/28 disposable or single use
• CPC Class Codes: C12M 41/26 of pH
• CPC Class Codes: C12M 41/32 of substances in solution
• CPC Class Codes: C12M 41/34 of gas
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404
• CPC Class Codes: C12M 41/40 of pressure
• CPC Class Codes: G01N 27/283 Means for supporting or introducing electrochemical probes
• CPC Class Codes: G01N 27/4165 for pH meters
• CPC Class Codes: G01N 33/00 Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

The present invention relates to a calibratable sensor unit for a reaction vessel, such as for example a fermenter (48), in particular a disposable fermenter, comprising at least one sensor device (14) to be calibrated and at least one compartment (26, 28, 30) containing a calibrating agent, the at least one sensor device and the at least one compartment (26, 28, 30) being accommodated movably relative to one another in a housing (12) that is connected to or can be connected to the reaction vessel (48), wherein the calibrating unit (10) is arranged to calibrate the at least one sensor device (14) by contact with the calibrating agent before chemical and/or physical parameters of a measurement substance are measured with the at least one sensor device (14). According to the invention it is proposed that the sensor device (10) is configured in such a way that the relative movement between the at least one sensor device (14) and the at least one compartment (26, 28, 30) can be carried out irreversibly.

Claims

1. A calibratable sensor unit for a reaction vessel, comprising:

at least one sensor device configured to be calibrated; and

at least one compartment containing a calibrating agent;

wherein the at least one sensor device and the at least one compartment are accommodated movably relative to one another in a housing configured to be connected to the reaction vessel;

wherein the calibratable sensor unit is arranged so as to calibrate the at least one sensor device by contact with the calibrating agent before chemical and/or physical parameters of a measurement substance are measured with the at least one sensor device;

wherein the at least one sensor device is further configured such that the relative movement between the at least one sensor device and the at least one compartment is irreversible; and

wherein the at least one sensor device is movable relative to the at least one compartment from an initial position in which the at least one sensor device does not contact the measurement substance, into a measurement readiness position in which the at least one sensor device is at least partially contactable by the measurement substance, wherein the measurement readiness position is different from the initial position.

2. The calibratable sensor unit according to claim 1, wherein the at least one sensor device, when in the initial position, is accommodated in a compartment containing a calibrating agent.

3. The calibratable sensor unit according to claim 1, wherein the housing surrounds the at least one sensor device in its initial position and the at least one compartment.

4. The calibratable sensor unit according to claim 1, wherein the at least one sensor device is automatically held when in the measurement readiness position.

5. The calibratable sensor unit according to claim 1, wherein the at least one compartment is closed by at least one septum.

6. The calibratable sensor unit according to claim 5, wherein the at least one septum is prepared for a penetration by the at least one sensor device.

7. The calibratable sensor unit according to claim 5, wherein the sensor device has a free end, which is implemented in such a way that the at least one septum of the at least one compartment can be penetrated by the free end of the sensor device.

8. The calibratable sensor unit according to claim 7, further comprising a plurality of compartments, which are arranged behind one another relative to the penetration direction of the sensor device and are separated from one another by respective septa arranged therebetween.

9. The calibratable sensor unit according to claim 7, further comprising a plurality of compartments which are arranged next to one another substantially orthogonal to the penetration direction.

10. The calibratable sensor unit according to claim 8, wherein at least one compartment of the plurality of compartments contains a cleaning agent.

11. The calibratable sensor unit according to claim 7, wherein a stripping device is associated with the at least one compartment, wherein the stripping device can be penetrated by the at least one sensor device and wherein the stripping device is implemented so that impurities, are retained in the respective compartment containing the calibrating agent or a cleaning agent.

12. The calibratable sensor unit according to claim 11, wherein the stripping device is formed by at least one septum or by at least one additional stripping means adjacent to the septum in the penetration direction.

13. The calibratable sensor unit according to claim 5, wherein the at least one compartment is formed as a tube-like sleeve, which has an elastic or rigid circumferential wall and is closed in the axial direction, at least on one side, by a septum.

14. The calibratable sensor unit according to claim 1, further comprising an actuating element configured to act on the at least one sensor device, wherein the actuating element is further configured to effect the irreversible relative movement between the at least one sensor device and the at least one compartment.

15. The calibratable sensor unit according to claim 8, wherein the calibrating agent and/or the cleaning agent are/is in the form of a sterilisable viscous medium.

16. The calibratable sensor unit according to claim 15, wherein the at least one calibrating agent contains synthetic polymers or natural polymers as thickening agents.

17. The calibratable sensor unit according to claim 1, wherein the reaction vessel is a fermenter.

18. The calibratable sensor unit according to claim 1, wherein the reaction vessel is a disposable fermenter.

19. The calibratable sensor unit according to claim 1, wherein a sensitive element of the at least one sensor device does not contact the measurement substance when the at least one sensor device is in the initial position, and wherein the sensitive element does contact the measurement substance when the at least one sensor device is in the measurement readiness position.

20. The calibratable sensor unit according to claim 2, wherein a sensitive element of the at least one sensor device is accommodated in a compartment containing a calibrating agent, when the at least one sensor device is in the initial position.

21. The calibratable sensor unit according to claim 1, wherein the at least one sensor device is automatically locked when in the measurement readiness position.

22. The calibratable sensor unit according to claim 5, wherein the at least one compartment is separated from an interior of the sensor unit enclosed by the housing, or from an adjacent compartment, or from the reaction vessel.

23. The calibratable sensor unit according to claim 5, wherein the at least one septum has regions of different material thickness so as to form a predetermined rupture point, and/or is at least partially perforated.

24. The calibratable sensor unit according to claim 5, wherein the least one compartment can be penetrated by the free end of the sensor device in a direction that is substantially orthogonal to the plane of the septum.

25. The calibratable sensor unit according to claim 11, wherein the stripping device is implemented so that the calibrating agent or the cleaning agent adhering to the at least one sensor device are retained in the respective compartment containing the calibrating agent or the cleaning agent.

26. The calibratable sensor unit according to claim 12, wherein the additional stripping means is a sealing ring.

27. The calibratable sensor unit according to claim 8, wherein the calibrating agent and/or the cleaning agent are/is a gel or paste.

28. The calibratable sensor unit according to claim 16, wherein the synthetic polymers are polyvinyl alcohol or hydroxyethylcellulose.

29. The calibratable sensor unit according to claim 16, wherein the natural polymers are polysaccharides.

30. The calibratable sensor unit according to claim 8, wherein each compartment of the plurality of compartments contains a cleaning agent.

31. The calibratable sensor unit according to claim 8, wherein the plurality of compartments is arranged such that the sensor device enters each compartment of the plurality of compartments successively.