Lighting design of high quality biomedical devices

• US 8,698,101 B2
• Filed: 10/23/2012
• Issued: 04/15/2014
• Est. Priority Date: 01/23/2009
• Status: Active Grant

• Current Assignee: Lumencor, Inc.
• Original Assignee: Lumencor, Inc.
• Inventors: Jaffe, Claudia B., Jaffe, Steven M., Conner, Arlie R.
• Primary Examiner(s): Porta, David
• Assistant Examiner(s): Maupin, Hugh H
• Application Number:
  US13/658,479
• Publication Number:
  US 20130099135A1
• Time in Patent Office: 539 Days
• Field of Search: 250/459.1
• US Class Current: 250/459.1
• CPC Class Codes: G01J 1/58 using luminescence generated by light
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G02B 6/0003 the light guides being doped with fluorescent agents

Abstract

The invention relates to a plurality of light sources to power a variety of applications including microarray readers, microplate scanners, microfluidic analyzers, sensors, sequencers, Q-PCR and a host of other bioanalytical tools that drive today'"'"'s commercial, academic and clinical biotech labs.

Claims

1. A light engine adapted to supply light to an optical instrument for analysis of a sample having one or more fluorescent tags, the light engine comprising:

a first solid state module, wherein the first solid state module includes at least one first solid state light source adapted to emit first light of a first color, and a first collimator adapted to receive the first light of the first color and generate a first collimated beam of light of the first color;

a second solid state module, wherein the second solid state module includes at least one second solid state light source adapted to emit second light of a second color different than the first color, and a second collimator adapted to receive the second light of the second color and generate a second collimated beam of light of the second color;

a third solid state module, wherein the third solid state module includes at least one third solid state light source adapted to emit third light of a third color different than the first color and the second color, and a third collimator adapted to receive the third light of the third color and generate a third collimated beam of light of the third color;

a fourth solid state module, wherein the fourth solid state module includes at least one fourth solid state light source adapted to emit fourth light of a fourth color different than the first color, second color and third color, and a fourth collimator adapted to receive the fourth light of the fourth color and generate a fourth collimated beam of light of the fourth color;

a fifth solid state module, wherein the fifth solid state module includes at least one fifth solid state light source adapted to emit fifth light of a fifth color different than the first color, second color, third color, and fourth color, and a fifth collimator adapted to receive the fifth light of the fifth color and generate a fifth collimated beam of light of the fifth color;

an optical output element adapted to couple the solid state light engine to an optical instrument; and

a plurality of dichroic elements adapted to direct each of the first collimated beam of light, second collimated beam of light, third collimated beam of light, fourth collimated beam of light, and fifth collimated beam of light, into the optical output element to generate an output light for transmission to said optical instrument wherein the output light is adapted to selectively excite the one or more fluorescent tags within the sample.

2. The light engine of claim 1, wherein the first solid state light source comprises:

a light pipe including a luminescent material;

a plurality of first LEDs which pump the luminescent material with excitation light having a color different than the first color; and

wherein, in response to said excitation light, the luminescent material of the light pipe emits said first light of said first color.

3. The light engine of claim 2, wherein the luminescent material of the light pipe emits said first light of said first color through a process of spontaneous emission.

4. The light engine of claim 3, wherein the light pipe comprises one of a glass doped with a rare earth metal, a glass activated with a transition metal, and a polymer doped with a dye.

5. The light engine of claim 2, further comprising:

a movable filter positionable in the first collimated beam of light to select the wavelength band of the first collimated beam of light reaching the output element.

6. The light engine of claim 2, wherein:

the at least one second solid state light source comprises a second LED adapted to emit second light of the second color;

the at least one third solid state light source comprises a third LED adapted to emit third light of the third color;

the at least one fourth solid state light source comprises a fourth LED adapted to emit fourth light of the fourth color; and

the at least one fifth solid state light source comprises a fifth LED adapted to emit fifth light of the fifth color.

7. The light engine of claim 1, wherein:

the at least one second solid state light source comprises a second LED adapted to emit second liaht of the second color;

the at least one third solid state light source comprises a third LED adapted to emit third light of the third color;

the at least one fourth solid state light source comprises a fourth LED adapted to emit fourth light of the fourth color; and

the at least one fifth solid state light source comprises a fifth LED adapted to emit fifth light of the fifth color.

8. The light engine of claim 1, further comprising:

at least one laser diode light source adapted to emit a sixth beam of light of a sixth color; and

wherein the plurality of dichroic elements are further adapted to direct the sixth beam of light into the optical output element to generate an output light for transmission to said optical instrument.

9. The light engine of claim 1, further comprising:

at least two laser diode light sources adapted to emit a sixth beam of light of a sixth color and a seventh beam of light of a seventh color; and

wherein the plurality of dichroic elements are further adapted to direct the sixth beam of light and seventh beam of light into the optical output element to generate an output light for transmission to said optical instrument.

10. The light engine of claim 1, wherein the first color, second color, third color, fourth color, and fifth color are selected from ultraviolet, blue, cyan, teal, green, orange and red.

11. The light engine of claim 1, wherein each solid state light module can be controlled independently.

12. The light engine of claim 1, wherein the light engine is capable of emitting narrowband light exceeding 500 mW per color with intensities up to 10 W/cm².

13. The light engine of claim 1, wherein each color can be switched independently in 30 μ

s or less.

14. A light engine adapted to supply light to an optical instrument for analysis of a sample having one or more fluorescent tags, the light engine comprising:

a first solid state module, wherein the first solid state module includes at least one first solid state light source adapted to emit first light of a first color, and a first collimator adapted to receive the first light of the first color and generate a first collimated beam of light of the first color;

a second solid state module, wherein the second solid state module includes at least one second solid state light source adapted to emit second light of a second color different than the first color, and a second collimator adapted to receive the second light of the second color and generate a second collimated beam of light of the second color;

a third solid state module, wherein the third solid state module includes at least one third solid state light source adapted to emit third light of a third color different than the first color and the second color, and a third collimator adapted to receive the third light of the third color and generate a third collimated beam of light of the third color;

a fourth solid state module, wherein the fourth solid state module includes at least one fourth solid state light source adapted to emit fourth light of a fourth color different than the first color, second color and third color, and a fourth collimator adapted to receive the fourth light of the fourth color and generate a fourth collimated beam of light of the fourth color;

a fifth solid state module, wherein the fifth solid state module includes at least one fifth solid state light source adapted to emit fifth light of a fifth color different than the first color, second color, third color, and fourth color, and a fifth collimator adapted to receive the fifth light of the fifth color and generate a fifth collimated beam of light of the fifth color;

an optical output element adapted to couple the solid state light engine to an optical instrument;

a plurality of dichroic elements adapted to direct each of the first collimated beam of light, second collimated beam of light, third collimated beam of light, fourth collimated beam of light, and fifth collimated beam of light, into the optical output element to generate an output light for transmission to said optical instrument wherein the output light is adapted to selectively excite the one or more fluorescent tags within the sample; and

wherein the first solid state light source comprises;

a light pipe including a luminescent material;

a plurality of first LEDs which pump the luminescent material with excitation light having a color different than the first color;

wherein, in response to said excitation light, the luminescent material of the light pipe emits said first light of said first color.

15. The light engine of claim 14, further comprising:

at least one laser diode light source adapted to emit a sixth beam of light of a sixth color; and

wherein the plurality of dichroic elements are further adapted to direct the sixth beam of light into the optical output element to generate an output light for transmission to said optical instrument.

16. The light engine of claim 14, further comprising:

at least two laser diode light sources adapted to emit a sixth beam of light of a sixth color and a seventh beam of light of a seventh color; and

wherein the plurality of dichroic elements are further adapted to direct the sixth beam of light and seventh beam of light into the optical output element to generate an output light for transmission to said optical instrument.

17. The light engine of claim 14, wherein the first color, second color, third color, fourth color, and fifth color are selected from ultraviolet, blue, cyan, teal, green, orange and red.

18. The light engine of claim 14, wherein each solid state light module can be controlled independently.

19. The light engine of claim 14, wherein the light engine is capable of emitting narrowband light exceeding 500 mW per color with intensities up to 10 W/cm².

20. A method for analyzing a sample comprising:

(a) providing excitation light from a first solid state module adapted to emit first light of a first color and a first collimator adapted to receive the first light of the first color and generate a first collimated beam of light of the first color, wherein the first solid state module includes a light pipe including a luminescent material, a plurality of first LEDs which pump the luminescent material with excitation light having a color different than the first color, wherein, in response to said excitation light, the luminescent material of the light pipe emits said first light of said first color;

(b) providing excitation light from a second solid state module, wherein the second solid state module includes at least one second solid state light source adapted to emit second light of a second color different than the first color, and a second collimator adapted to receive the second

(c) providing excitation light from a third solid state module, wherein the third solid state module includes at least one third solid state light source adapted to emit third light of a third color different than the first color and the second color, and a third collimator adapted to receive the third light of the third color and generate a third collimated beam of light of the third color;

(d) providing excitation light from a fourth solid state module, wherein the fourth solid state module includes at least one fourth solid state light source adapted to emit fourth light of a fourth color different than the first color, second color and third color, and a fourth collimator adapted to receive the fourth light of the fourth color and generate a fourth collimated beam of light of the fourth color;

(e) providing excitation light from a fifth solid state module, wherein the fifth solid state module includes at least one fifth solid state light source adapted to emit fifth light of a fifth color different than the first color, second color, third color, and fourth color, and a fifth collimator adapted to receive the fifth light of the fifth color and generate a fifth collimated beam of light of the fifth color;

(f) providing excitation light from at least one laser diode light source;

(g) combining the excitation light from the first solid state module, second solid state module, third solid state module, fourth solid state module, and fifth solid state module and the excitation light from the at least one laser diode light source using a plurality of dichroic elements to produce a beam of excitation light having a plurality of colors;

(h) directing the beam of excitation light onto the sample; and

(i) detecting fluorescent light emitted from the sample in response to absorption by the sample of one or more of said plurality of colors of the beam of excitation light.