Droplet actuator with improved top substrate

• US 8,702,938 B2
• Filed: 09/04/2008
• Issued: 04/22/2014
• Est. Priority Date: 09/04/2007
• Status: Active Grant

• Current Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Original Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Inventors: Srinivasan, Vijay, Pollack, Michael G., Shenderov, Alexander, Hua, Zhishan, Sudarsan, Arjun
• Primary Examiner(s): DIETERLE, JENNIFER M
• Application Number:
  US12/676,384
• Publication Number:
  US 20100282608A1
• Time in Patent Office: 2,056 Days
• Field of Search: 204/450, 204/600, 422/504, 422/68.1, 422/82.01
• US Class Current: 204/450
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension
• CPC Class Codes: Y10T 29/49826 Assembling or joining

Abstract

The invention provides a droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein: (a) the base substrate comprises electrodes configured for conducting droplet operations in the gap; and (b) the top substrate comprises a first portion coupled to second portion, where the second portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.

Claims

1. A droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein:

(a) the base substrate comprises electrodes configured for conducting droplet operations in the gap;

(b) the top substrate comprises a first substrate portion coupled to a second frame portion, where the second portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap, wherein the first substrate portion and second frame portion are not made of the same material; and

(c) one or more openings formed in the second frame portion of the top substrate establishing a fluid path extending from an exterior of the droplet actuator and into the gap.

2. The droplet actuator of claim 1 wherein the first substrate portion comprises a more uniformly planar surface exposed to the gap than the second frame portion.

3. The droplet actuator of claim 1 wherein the first substrate portion is more transparent than the second frame portion.

4. The droplet actuator of claim 1 wherein the first substrate portion is transparent, and the second frame portion is opaque.

5. The droplet actuator of claim 1 wherein the first substrate portion is harder than the second frame portion.

6. The droplet actuator of claim 1 wherein the first substrate portion is more thermally stable than the second frame portion.

7. The droplet actuator of claim 1 wherein the first substrate portion is more resistant to damage caused by temperature fluctuation than the second frame portion.

8. The droplet actuator of claim 1 wherein the base substrate comprises a printed circuit board (PCB) substrate.

9. A method of loading a fluid onto a droplet actuator, the method comprising providing a droplet actuator of claim 1 and loading a fluid through the opening and into the gap.

10. A method of conducting a droplet operation, the method comprising:

(a) providing a droplet actuator of claim 1;

(b) loading a liquid onto the droplet actuator into proximity with one or more electrodes; and

(c) using the one or more electrodes to conduct the droplet operation.

11. A droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein:

(a) the base substrate comprises electrodes configured for conducting droplet operations in the gap; and

(b) the top substrate comprises a glass substrate portion coupled to a non-glass frame portion, where the non-glass frame portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap.

12. The droplet actuator of claim 11 wherein the non-glass frame portion comprises a plastic or resin portion.

13. The droplet actuator of claim 11 wherein the non-glass frame portion comprises a frame into which the glass substrate portion is inserted.

14. The droplet actuator of claim 11 wherein the fluid path is arranged to flow fluid into an actual or virtual reservoir associated with one or more reservoir electrodes associated with the base substrate.

15. The droplet actuator of claim 11 wherein the fluid path is arranged to flow fluid into proximity with one or more of the electrodes.

16. The droplet actuator of claim 11 wherein the glass substrate portion does not include openings therein.

17. The droplet actuator of claim 11 wherein:

(a) the non-glass frame portion overlaps the glass substrate portion, and

(b) an aperture is provided in the non-glass frame portion for providing a sensing path from the gap, through the glass substrate portion, through the aperture to an exterior of the droplet actuator.

18. The droplet actuator of claim 17 further comprising a fitting provided in association with the aperture for fitting a sensor onto the droplet actuator.

19. The droplet actuator of claim 17 further comprising a handle extending from the glass substrate portion and arranged to facilitate user handling of the droplet actuator.

20. The droplet actuator of claim 11 wherein the non-glass frame portion further comprises a hinged cover arranged to seal the openings when the hinged cover is in a closed position.

21. The droplet actuator of claim 20 wherein the hinged cover comprises one or more dried reagents associated therewith, such that when fluid is present in one or more of the openings, and the cover is closed, the dried reagents contact the fluid and are combined therewith to form fluid reagents.

22. The droplet actuator of claim 11 wherein:

(a) the non-glass frame portion overlaps the glass substrate portion; and

(b) one or more of the openings extends through the non-glass frame portion, through the glass substrate portion, and into the gap.

23. The droplet actuator of claim 22 wherein the opening extending through the non-glass frame portion is configured as a fluid reservoir.

24. A method of assembling the droplet actuator of claim 11, the method comprising:

(a) coupling the glass substrate portion to the non-glass frame portion; and

(b) assembling the top substrate with the bottom substrate to form a gap therebetween suitable for conducting droplet operations.