Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

  • US 8,703,928 B2
  • Filed: 09/09/2011
  • Issued: 04/22/2014
  • Est. Priority Date: 02/09/1996
  • Status: Expired due to Fees
First Claim
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1. An instrument comprising:

  • a solid support comprising at least 25 types of capture oligonucleotides immobilized on the solid support at an array of positions, wherein each type of capture oligonucleotide is greater than 16 nucleotides and differs in nucleotide sequence, when aligned to another type of capture oligonucleotide that is located on an adjacent position of said solid support, by at least 25%;

    one or more nucleic acid molecules hybridized to complementary portions of the one or more capture oligonucleotides on said solid support, wherein the capture oligonucleotides hybridize to the complementary portions of the nucleic acid molecules under uniform hybridization conditions; and

    an imager configured to detect the hybridized nucleic acid molecules.

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