Protein purification

CAFC
  • US 8,710,196 B2
  • Filed: 10/04/2011
  • Issued: 04/29/2014
  • Est. Priority Date: 09/11/2002
  • Status: Active Grant
First Claim
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1. A method for purifying an antibody from a composition comprising the antibody and a contaminant, which method comprises the following steps performed sequentially:

  • (a) binding the antibody to a cation exchange material with an equilibration buffer at a first conductivity;

    (b) washing the cation exchange material with a wash buffer, wherein the conductivity of the wash buffer increases from a second conductivity that is higher than the first conductivity to a third conductivity during the washing;

    (c) passing a fixed volume of wash buffer at the third conductivity over the cation exchange material; and

    (d) eluting the antibody from the cation exchange material with an elution buffer at a fourth conductivity that is higher than the third conductivity.

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