Microtitration plate

• US 8,728,415 B2
• Filed: 05/24/2011
• Issued: 05/20/2014
• Est. Priority Date: 03/22/2002
• Status: Active Grant

• Current Assignee: Eppendorf Se
• Original Assignee: Eppendorf AG
• Inventors: Seippel, Martin
• Primary Examiner(s): Hyun, Paul
• Application Number:
  US13/114,503
• Publication Number:
  US 20120129250A1
• Time in Patent Office: 1,092 Days
• Field of Search: 422/553
• US Class Current: 422/553
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B29C 2045/1659 Fusion bonds
• CPC Class Codes: B29C 45/16 Making multilayered or multicoloured articles B29C45/0062 takes precedence; feeding colouring materials into the injection unit B29C45/1816

Abstract

A microtitration plate for use for automated polymerase chain reaction (PCR) is provided. The microtitration plate includes a frame which has a plate with a multiplicity of holes molded of a polypropylene or a polycarbonate. The microtitration plate further includes a multiplicity of receptacles made of the same polypropylene or polycarbonate. The receptacles are fixedly connected to the plate at the holes by fusing the same polypropylene or polycarbonate thereby directly molding the receptacles to the plate Each receptacle has a receiving portion protruding from the underside of the plate, and is accessible from the upper surface of the plate through apertures.

Claims

1. A microtitration plate for use for automated polymerase chain reaction (PCR), comprising:

a frame (2) which has a plate (4) wherein the plate (4) has a multiplicity of holes (6′

) molded of a polypropylene; and

a multiplicity of thin-wall receptacles (3) having a wall thickness of from about 0.05 to about 0.25 mm at least in one wall portion, and made of identical same type of polypropylene as the frame (2) and the plate (4), with the receptacles (3) fixedly connected to the plate (4) at the holes (6′

) by fusing to each other the identical type of polypropylene of the receptacles (3) and the plate (4) due to direct molding of the receptacles (3) to the plate (4), the receptacles (3) having receiving portions (9, 10, 11) protruding from the underside (8) of the plate (4), and are accessible from the upper surface (7) of the plate (4) through apertures (15), wherein the frame (2) and the receptacles (3) are composed of the identical type of polypropylene.

2. The microtitration plate according to claim 1, wherein the receptacles (3) have a wall portion (10) of a very small wall thickness adjacent to a receptacle bottom (9) and have an upper wall portion (12) connected to the plate (4).

3. The microtitration plate according to claim 1, wherein the receptacles (3) have a collar of an increased wall thickness as an upper wall portion (12) connected to the plate (4).

4. The microtitration plate according to claim 1, wherein each receptacle (3) has a substantially cup-shaped bottom (9), a wall portions (10) of a small wall thickness that is substantially conical, and a wall portion (11) of a wall thickness which gradually increases upwardly adjoining the wall portion (10) of a small wall thickness and an upper wall portion (12) connected to the plate (4) and extending to an opposing side of the plate (4).

5. The microtitration plate according to claim 1, wherein the receptacles (3) have molding points at the bottom (9) of the receptacles.

6. The microtitration plate according to claim 1, wherein the frame (2) has a bordering (5) protruding from the underside (8) thereof at the edge of the plate (4).

7. The microtitration plate according to claim 1, wherein the frame (2) has several edge-sided molding points.

8. The microtitration plate according to claim 1, which is made of a plastic including a filler which does not contain protein.

9. The microtitration plate according to claim 1, which is made of a plastic filled with fiberglass.

10. A microtitration plate for use for automated polymerase chain reaction (PCR), comprising:

a frame (2) which has a plate (4) wherein the plate (4) has a multiplicity of holes (6′

) molded of a polycarbonate

a multiplicity of thin-wall receptacles (3) having a wall thickness of from about 0.05 to about 0.25 mm at least in one wall portion, and molded of a polycarbonate, with the receptacles (3) fixedly connected to the plate (4) at the holes (6′

) by fusing to each other both the polycarbonate of the plate (4) and the polycarbonate of the receptacles (3) due to direct molding of the receptacles (3) to the plate (4), the receptacles (3) having receiving portions (9, 10, 11) protruding from the underside (8) of the plate (4), and are accessible from the upper surface (7) of the plate (4) through apertures (15), wherein the frame (2) and the receptacles (3) are composed of polycarbonates.