Reprogramming T cells and hematopoietic cells

  • US 8,741,648 B2
  • Filed: 06/04/2010
  • Issued: 06/03/2014
  • Est. Priority Date: 06/05/2009
  • Status: Active Grant
First Claim
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1. A method for producing human induced pluripotent stem cells from CD34+ hematopoietic progenitor cells comprising the steps of:

  • (a) obtaining a cell population comprising human CD34+ hematopoietic progenitor cells, wherein the CD34+ hematopoietic progenitor cells are from a subject whose cells have not been mobilized; and

    (b) producing iPS cells from the CD34+ hematopoietic progenitor cells of the cell population through the introduction of nucleic acids encoding reprogramming factors selected from Oct4, Sox2, cMyc, Klf4, Nanog and Lin28, the nucleic acids being comprised in one or more episomal vectors, and culturing the cells under defined, feeder-free conditions to provide a human iPS cell population that is essentially free of integrated, exogenous viral elements.

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